Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-11-17
1998-07-21
Leary, Louise
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 732, 435 8, 435 4, 435 29, 435 30, 435 34, 435 16, 435 18, 435968, 4352831, 4352883, 4352887, 436172, 422 50, 422 52, G01N 3353, G01N 33554, C12Q 100, C12Q 102
Patent
active
057833990
ABSTRACT:
A method which utilize chemiluminescence for analyte detection and the detection of bacteria on surfaces. The method uses a device comprising a sampling portion made of a first adsorbent material, a container, and a second adsorbent material located within the container. The sampling portion collects analytes from a test sample which includes a surface or volume of a liquid. The second adsorbent material holds one or more chemiluminescent components including luciferase enzyme and cofactors in a dry state. In a preferred embodiment, the sampling portion is swabbed over a suspected contaminated surface. A bacteriolytic solution is then added to the adsorbent and releases ATP from any bacteria present. The ATP diffuses into the second adsorbent and mixes with the reagents therein to produce detectable light.
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patent: 5366867 (1994-11-01), Kawakami et al.
DeLuca, M., et al. "Factors Affecting the Kinetics Of Light Emission From Crude and Purified Firefly Luciferase," Anallytical Biochem. 95:194-98 (1979) Month not available.
Bernstein David
Childs Mary Ann
Gray Erick
Shipman Gregory K.
Trainor William P.
Leary Louise
Universal Healthwatch, Inc.
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