Chemiluminescence method of 1,2-dioxetane and composition...

Details Chemiluminescence method of 1,2-dioxetane and composition... Chemiluminescence method of 1,2-dioxetane and composition...

2006-08-15

2006-08-15

Ceperley, Mary E. (Department: 1641)

Chemistry: analytical and immunological testing

Involving producing or treating antigen or hapten

Producing labeled antigens

C436S172000, C436S904000, C435S968000, C435S021000, C435S028000, C549S507000, C549S497000, C549S504000

Reexamination Certificate

active

07091051

ABSTRACT:
A chemiluminescence method characterized in that when a 1,2-dioxetane derivative of the formula 1:wherein each of R1, R2, R3, R4and R5which are independent of one another, is a hydrogen atom, an alkyl group or an aryl group, or each pair of R2and R3, and R4and R5, which are independent of each other, may form together a cyclic alkyl group, R6is a hydroxyl group, an alkoxyl group, an aralkyloxy group, a group represented by —OSi(R8R9R10) (provided that each of R8, R9and R10which are independent of one another, is an alkyl group or an aryl group), a phosphate group or a group represented by S(C═O)R11(provided that R11is an alkyl group or an aryl group), and R7is a hydrogen atom, a halogen atom, an alkyl group or an alkoxyl group, is let generate chemiluminescence by means of an activator selected from the group consisting of a base, an acid, a salt, a fluorine compound, an enzyme, a catalyst and an amine compound, a cationic surfactant and a fluorescent material are made to coexist.

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