Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1995-05-30
1998-12-08
Richter, Johann
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
549332, 549222, 435 19, 435 21, C07D30514, C12Q 142, C12Q 144
Patent
active
058467530
ABSTRACT:
A method of detecting the presence of a substance being monitored in a medium, selected from the group of substances including organophosphorus compounds and the metal ions Zn, Be and Bi, including the steps of: providing a 1,2-dioxetane phenyl phosphate compound; providing a phosphatase that catalytically degrades the 1,2-dioxetane phenyl phosphate compound to produce light, the catalytic activity of the phosphatase toward 1,2-dioxetane phenyl phosphate compound being altered by the substance being monitored; exposing the 1,2-dioxetane phenyl phosphate compound and the phosphatase together to a medium which may contain the substance being monitored; detecting light produced after the exposing step; and determining, from the detected light, the presence and concentration in the medium of the substance being monitored.
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Akkara Joseph A.
Ayyagari Madhu S. R.
Kamtekar Sanjay
Kaplan David L.
Kumar Jayant
Cross Lutz Laura R.
Ranucci Vincent J.
Richter Johann
The United States of America as represented by the Secretary of
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