Chemiluminescence assays using stabilized dioxetane derivatives

Details Chemiluminescence assays using stabilized dioxetane derivatives Chemiluminescence assays using stabilized dioxetane derivatives

1989-07-19

1992-03-10

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 79, 435 19, 435 21, 436172, 436800, 436805, C12Q 168, C12Q 142, G01N 2176

Patent

active

050949390

ABSTRACT:
A multi-pH chemiluminescence-based assay method comprising first-stage light production by the activation by cleavage of a phosphatase cleavable dioxetane derivative within a pH range optimal for enzyme catalysis, followed by the second-stage generation of increased light energy from the products of enzyme-catalyzed cleavage of the dioxetane derivative by the adjustment of the pH to a strongly alkaline pH optimal for the generation of light energy. The assay method is suitable for both solution phase and solid state assay formats. Light generation enhancers can be used further to increase the light yield.

REFERENCES:
patent: 4778755 (1988-10-01), Tsay et al.
Schaap, A. P., et al., Tet. Letters, 28:935 (1987).
Schaap, A. P., et al., Tet. Letters, 28:1159 (1987).
Bronstein, I. Y., et al., Clin. Chem., 35(9):1441 (1989).

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