Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1995-03-15
1996-12-31
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 18, 435 19, C12Q 100, C12Q 134, C12Q 144
Patent
active
055893287
ABSTRACT:
Chemiluminescence-based assays that detect or quantify enzymes that catalyze the hydrolysis of indoxyl esters are provided. The assays are based on the hydrolysis of indoxyl esters by enzymes of interest, such as alkaline phosphatase and others that are used as labels in immunoassays or nucleic acid hybridization reactions, or are present in body fluids. The assays include the steps of reacting a test sample with an indoxyl ester and, then, immediately or within a short time, typically less than about fifteen minutes, measuring the resulting chemiluminescence. The resulting chemiluminescence may be amplified by adding a chemiluminescence-amplifying reagent, such as horseradish peroxidase or lucigenin to the reaction.
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Fish Robert D.
Gitomer Ralph
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