Chemically wired fructose dehydrogenase electrodes

Chemistry: electrical and wave energy – Apparatus – Electrolytic

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20415312, 435 26, 435817, 436806, G01N 27327

Patent

active

052981444

ABSTRACT:
An enzyme electrode is disclosed for use in voltammetric systems wherein a PQQ dehydrogenase enzyme is chemically bonded to a water soluble polyvinylpyridine osmium bipyridine polymer. The enzyme and polymer are adsorbed onto the surface of the electrode with the polymer providing direct electron transfer from enzyme to electrode and vice-versa without the aid of additional mediators or electron shuttles added to the system.

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cis-BIS(2,2'-Bipyridine-N,N') Complexes of Ruthenium(III) (II) and Osmium (III)(II), Lay, P. A., Sargeson, A. M.; Taube, H., Inorg. Syn, 1986, 24, 291-306.
"Development of an On-line Glucose Sensor for Fermentation Monitoring," Brooks, S. L. et al., Biosensors 3 (1987/88) 45-56.
"D-Fructose Dehydrogenase of Gluconobacter Industrius: Purification, Characterization, & Appln. to Enzymatic Microdetermination of D-Fructose," Ameyama, M. et al., J. of Bacteriology, Feb. 1981, pp. 814-823.
"Electrical Wiring of Redox Enzymes," Heller, A., Accts. of Chemical Research, 1990, 23 pp. 128-134.
"Redox Polymer Films Containing Enzymes," Gregg, B. A. et al., J. Phys. Chem. 1991, 95, pp. 5976-5980.
"Redox Polymer Films Containing Enzymes," Gregg, B. A. et al., J. Phys. Chem. 1991, 95, pp. 5970-5975.

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