Chemically targeted positional identification of...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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C435S023000, C435S069700, C530S402000, C530S412000, C530S413000, C930S020000

Reexamination Certificate

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10938371

ABSTRACT:
Methods are provided for determining the position of a post-translationally modified (PTM) amino acid residue such as a phosphorylated amino acid residue or an O-glycosylated amino acid residue in a peptide. Also provided are methods for determining the identity of a PTM amino acid residue in a peptide. In addition, kits for practicing such methods are provided.

REFERENCES:
Jeno et al., Interna Sequences from proteins digesed in polyacrylamide gels. Anal. Chem., 224, 75-82, 1995.
Morpugo et al., N-hydroxysuccinimide carbonates and carbamates are useful reactive reagents for coupling ligands to lysines on proteins., J. Biochem. Byophys methods, 38, 17-28, 1999.
Carr et al., “Selective Detection and Sequencing of Phosphopeptides at the Femtomole Level by Mass Spectrometry,”Analytical Biochemistry, vol. 239: pp. 180-192 (1996).
Catsimpoolas and Wood, “Specific Cleavage of Cystine Peptides by Cyanide,”The Journal of Biological Chemistry, vol. 241, No. 8: pp. 1790-1796 (1966).
Cole, David, “S-Aminoethylation,”Methods in Enzymology, vol. 11: pp. 315-317 (1967).
Gerken et al., “Determination of the Site-specific O-Glycosylation Pattern of the Porcine Submaxillary Mucin Tandem Repeat Glycopeptide,”The Journal of Biological Chemistry, vol. 272, No. 15: pp. 9709-9719 (1997).
Greis et al., “Selective Detection and Site-Analysis of O-GlcNAc-Modified Glycopeptides by β-Elimination and Tandem Electrospray Mass Spectrometry,”Analytical Biochemistry, vol. 234: pp. 38-49 (1996).
Havlis et al. “Fast-Response Proteomics by Accelerated In-Gel Digestion of Proteins,”Anal. Chem. vol. 75: pp. 1300-1306 (2003).
Helmbrecht et al., “Chaperones in cell cycle regulation and mitogenic signal transduction: a review,”Cell Prolif. vol. 33:pp. 341-365 (2000).
Huang et al., “Matrix-assisted laser desorption/ionization mass spectrometry compatible β-elimination of O-linked oligosaccharides,”Rapid Communications in Mass Spectrometry, vol. 16: pp. 1199-1204 (2002).
Hunziker et al., “Peptide Fragmentation Suitable for Solid-Phase Microsequencing,”Biochem. J., vol. 187: pp. 515-519 (1980).
Kawata et al., “Amino-acid sequence of ribonuclease T2fromAspergillus oryzae,” Eur. J. Biochem., vol. 176: pp. 683-697 (1988).
Masaki et al., “Hydrolysis of S-2 Aminoethylcysteinyl Peptide Bond byAchromobacterProtease I,”Biosci. Biotech. Biochem., vol. 58, No. 1: pp. 215-216 (1994).
Meyer et al., “Sequence analysis of phosphoserine-containing peptides,”FEBS Letters, vol. 204, No. 1: pp. 61-66 (1986).
Rademaker et al., “Mass Spectrometric Determination of the Sites of O-Glycan Attachment with Low Picomolar Sensitivity,”Analytical Biochemistry, vol. 257: pp. 149-160 (1998).
Rall et al., “The Amino Acid Sequence of Ferredoxin fromClostridium acidi-urici,” Biochemistry, vol. 8, No. 6: pp. 2486-2496 (1969).
Rusnak et al., “Identification of Phosphorylated and Glycosylated Sites in Peptides by Chemically Targeted Proteolysis,”Journal of Biomolecular Techniques, vol. 13, No. 4: pp. 228-237 (2002).
Schwartz et al., “N-(β-lodoethyl)trifluoroacetamide: A New Reagent for the Aminoethylation of Thiol Groups in Proteins,”Analytical Biochemistry, vol. 106: pp. 43-48 (1980).
Steen and Mann, “A New Derivazation Strategy for the Analysis of Phosphopeptides by Precursor Ion Scanning in Positive Ion Mode,”J. Am. Soc. Mass Sepctrom. vol. 13: pp. 996-1003 (2002).
Thompson et al., “Characterization of Protein Phosphorylation by Mass Spectrometry Using Immobilized Metal Ion Affinity Chromatography with On-Resin β-Elimination and Michael Addition,”Anal. Chem., vol. 75: pp. 3232-3243 (2003).
Tomita and Marchesi, “Amino-acid sequence oligosaccharide attachment sites of human erythrocyte glycophorin,”Proc. Nat. Acad. Sci. USA, vol. 72, No. 8: pp. 2964-2968 (1975).

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