Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-05-24
1997-07-22
Moody, Patricia R.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 245, 530370, 530379, 435 691, 4351723, 4352404, 4353201, 800205, C12N 1529, C12N 1582, A01H 500
Patent
active
056505051
ABSTRACT:
The present invention provides chemically regulatable DNA sequences capable of regulating transcription of an associated DNA sequence in plants or plant tissues, chimeric constructions containing such sequences, vectors containing such sequences and chimeric constructions, and transgenic plants and plant tissues containing these chimeric constructions. In one aspect, the chemically regulatable DNA sequences of the invention are derived from the 5' region of genes encoding pathogenisis-related (PR) proteins. The present invention also provides anti-pathogenic sequences derived from novel cDNAs coding for PR proteins which can be genetically engineered and transformed into plants to confer enhanced resistance to disease. Also provided is a method for the exogenous regulation of gene expression in plants, which comprises obtaining a plant incapable of regulating at least one gene or gene family, or at least one heterologous gene, due to the deactivation of at least one endogenous signal transduction cascade which regulates the gene in the plant, and applying a chemical regulator to the plant at a time when expression of the gene is desired. A novel signal peptide sequence and corresponding DNA coding sequence is also provided. Further provided are assays for the identification and isolation of additional chemically regulatable DNA sequences and cDNAs encoding PR proteins and assays for identifying chemicals capable of exogenously regulating the chemically regulatable DNA sequences of the invention.
REFERENCES:
patent: 4394443 (1983-07-01), Weissman et al.
patent: 4399216 (1983-08-01), Axel et al.
patent: 4634665 (1987-01-01), Axel et al.
patent: 4886753 (1989-12-01), Marcker et al.
patent: 4940840 (1990-07-01), Suslow et al.
Abel et al., "Delay of Disease Development in Transgenic Plants that Express the Tobacco Mosaic Virus Coat Protein Gene", Science, 232:738-743 (1986).
Alexander et al., "Increased tolerance to two comycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a", Proc. Natl. Acad. Sci., USA, 90: 7327-7331 (1993).
Antoniw et al., "The role of pathogenesis-related proteins", Plant Resistance to Viruses, 57-91 Ciba Foundation Symposium 133, Wiley and Sons (1987) pp. 57-71.
Asselin et al., "Light-influenced extracellular accumulation of b (pathogenesis-related) proteins in Nicotiana green tissue induced by various chemicals or prolonged floating on water" Can. J. Bot., 63:1276-1283 (1985).
Beilmann et al., "Functional analysis of the PR-1a promoter by transient and by stable transfections", Abstract International Meeting on Biology, Valencia, Spain, Oct. 11-26, 1989.
Bell et al., "Differential Accumulation of Plant Defence Gene." Mol. Cell. Biol., 6:1615-1623 (1986).
Bol et al., "The synthesis and possible functions of virus-induced proteins in plants", Microbiol. Sci., 5:47-52 (1988).
Bol et al., "Characterization of pathogenesis-related proteins and genes", CIBA Symp., 133:72-91 (1987).
Bol et al., "Plant defense genes induced by virus infection", J. Cell Biochem., 12C:244 (1988).
Broglie et al., "Ethylene-regulated gene expression: Molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris", Proc. Natl. Acad. Sci. USA, 83:6820-6824 (1986).
Carr et al., "Synthesis of pathogenesis-related proteins in tobacco is regulated at the level of mRNA accumulation and occurs on membrane-bound polysomes", Proc. Natl. Acad. Sci., USA 82:7999-8003 (1985).
Comai et al., "Novel and useful properties of a chimeric plant promoter combining CaMV 35S and MAS elements", Pl Molec Biol 15: 373-381 (1990).
Cornelissen et al., "Molecular characterization of messenger RNAs for `pathogenesis-related` proteins 1a, 1b and 1c, induced by TMV infection of tobacco", The EMBO Journal, 5:37-40 (1986).
Cornelissen et al., "A tobacco mosaic virus-induced tobacco protein is homologous to the sweet-tasting protein thaumatin", Nature, 321:531-532 (1986).
Cornelissen et al., "Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group", Nucleic Acids Research, 15:6799-6811 (1987).
Cutt et al., "Pathogenesis-Related Proteins of Nicotiana tabacum" J. Cell. Biochem. Suppl. 12C, Y210 (1988).
Duguid et al., "Isolation of cDNAs of scrapie-modulated RNAs by subtractive hybridization of a cDNA library", Proc. Natl. Acad. Sci. USA, 85:5738-5742 (1988).
Ellis et al., "Maize Adh-1 promoter sequences control anaerobic regulation: addition of upstream promoter elements from constitutive genes is necessary for expression in tobacco", EMBO J., 6:11-16 (1987).
Fraser, "Are `Pathogenesis-related` Proteins Involved in Acquired Systemic Resistance of Tobacco Plants to Tobacco Mosaic Virus?", Virol., 58:305-313 (1982).
Gelvin et al., "Use of a T.sub.R T-DNA promoter to express genes in plants and bacteria", Mol. Gen Genet, 199:240-248 (1985).
Gianinazzi, "Genetic and Molecular Aspects of Resistance Induced by Infections or Chemicals", Plant Response to the Environment pp. 321-342 (MacMillan Publishing Company, London, 1984).
Gianinazzi et al., "The genetic and molecular basis of b-proteins in the genus Nicotiana", Neth. J. Pl. Path., 89: 275-281 (1983).
Hedrick et al., "Chitinase cDNA Cloning and mRNA Induction" Plant Physiol., 86:182-186 (1988).
H.o slashed.j et al., "Purification of (1.fwdarw.3)-.beta.-glucan endohydrolase isoenzyme II from germinated barley and determination of its primary structure from a cDNA clone", Plant Molecular Biology, 13:31-42 (1989).
Hooft van Huijsduijnen et al, "Virus-induced synthesis of messenger RNAs for precursors of pathogenesis-related proteins in tobacco", The EMBO Journal 4:2167-2171 (1985).
Hooft van Huijsduijnen et al., "cDNA cloning of six mRNAs induced by TMV infection of tobacco and a characterization of their translation products", EMBO J., 5:2057-2061 (1986).
Hooft van Huijsduijnen et al., "Homology between chitinases that are induced by TMV infection of tobacco", Plant Molecular Biology, 9:411-420 (1987).
Horowitz et al., "TMV induced gene expression in tobacco", Journal of Cellular Biochemistry, 11B:37 Abstract F320, UCLA Symposia on Molecular & Cellular Biology, (Feb. 2-27, 1987).
Jackson et al., "Construction of a yeast vector directing the synthesis and release of barley (1.fwdarw.3, 1.fwdarw.4)-.beta.-glucanase.", Biochem. Genetics, 107:187 Abstract 107:18833M (1987).
Jamet et al., "Purification and characterization of 8 of the pathogenesis-related proteins in tobacco leaves reacting hypersensitively to tobacco mosaic virus", Plant Molecular Biology 6:69-80 (1986).
Jefferson et al., "Beta-glucuronidase (GUS) as a sensitive and versatile gene fusion marker in plants", J. Cell. Biol., 11B:57 (1987).
Jefferson et al., "GUS fusions: .beta.-glucuronidase as a sensitive and versatile gene fusion marker in higher plants", The EMBO Journal, 6:3901-3907 (1987).
Kauffmann et al., "Biological function of `pathogenesis-related` proteins: four PR proteins of tobacco have 1,3-.beta.-glucanase activity", The EMBO Journal 6:3209-3212 (1987).
Kay, et al., "Duplication of CaMV 35S Promoter Sequences Creates a Strong Enhancer for Plant Genes", Science, 236:1299-1302 (1987).
Koncz, et al., "The promoter of T.sub.L -DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector", Mol Gen Genet 204:383-396 (1986).
Kowalski et al., "Vectors for the direct selection of cDNA clones corresponding to mammalian cell mRNA of low abundance", Gene, 35:45-54 (1985).
Lamb et al., "Signals and Transduction Mechanisms for Activation of Plant Defenses against Microbial Attack", Cell, 56:215-224 (1989).
Lawton et al., "The Molecular Biology of Systemic Acquired Resistance", Development in Plant Pathology, 2:422-432 (1993).
Legrand et al., "Biological function of pathogenesis-related proteins: Four tobacco pathogenesis-related proteins are chitinases", Proc. Natl. Acad. Sci. USA 84:6750-6754 (1987).
Linthorst et al., "Constitutive Expression of Pathogenesis-Related Proteins PR-1, GRP, and PR-S in Tobacco Has No Effect on Virus Infection", The Plant Cell, 1:285-291 (1989).
Lucas
Alexander Danny C.
Beck James J.
Duesing John H.
Friedrich Leslie B.
Goodman Robert M.
Meigs J. Timothy
Moody Patricia R.
Novartis Corporation
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