Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.
Patent
1997-10-27
2000-12-26
Ulm, John
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Lymphokines, e.g., interferons, interlukins, etc.
514 2, 530350, C07K 14535
Patent
active
06166183&
ABSTRACT:
The present invention provides a chemically-modified protein prepared by binding polyethylene glycol to a polypeptide characterized by being the product of expression by a host cell of an exogenous DNA sequence and substantially having the following amino acid sequence: (Het)n - Thr Pro Leu Gly Pro Ala Ser Ser Leu Pro Gln - Ser Phe Leu Leu Lys Cys Leu Glu Gln Val Arg - Lys Ile Gln Gly Asp Gly Ala Ala Leu Gln Glu - Lys Leu Cys Ala Thr Tyr Lys Leu Cys His Pro - Glu Glu Leu Val Leu Leu Gly His Ser Leu Gly - Ile Pro Trp Ala Pro Leu Ser Ser Cys Pro Ser - Gln Ala Leu Gln Leu Ala Cly Cys Leu Ser Gln - Leu His Ser Gly Leu Phe Leu Tyr Gln GIY Leu - Leu Gln Ala Leu Glu Gly Ile Ser Pro Glu Leu - Gly Pro Thr Leu Asp Thr Leu Gln Leu Asp Val - Ala Asp Phe Ala Thr Tbr Ile Trp Gln Gln Het - Glu Glu Leu Gly Het Ala Pro Ala Leu Gln Pro - Thr Gln Gly Ala Het Pro Ala Phe Ala Ser Ala - Phe Gln Arg Arg Ala Gly Gly Val Leu Val Ala - Ser His Leu Gln Ser Phe Leu Glu Val Scr Tyr - Arg Val Leu Arg His Leu Ala Gln Pro (n = 0 or 1)
The chemically-modified protein according to the present invention has a neutrophils-increasing activity much more lasted than that of the intact human G-CSF, enabling fewer numbers of administration with a lower dose.
REFERENCES:
patent: 4179337 (1979-12-01), Davis et al.
patent: 4766106 (1988-08-01), Katre et al.
patent: 4791192 (1988-12-01), Nakagawa et al.
patent: 4810643 (1989-03-01), Souza
patent: 4833127 (1989-05-01), Ono et al.
patent: 4847325 (1989-07-01), Shadle et al.
Goodson et al., "Site-directed Pegylation of Recombinant Interleukin-2 at its Glycosylation Site," Bio/Technology 8:343-346 (1990.
Habeeb et al., "Determination of Free Amino Goups in Proteins by Trinitrobenzenesulfonic Acid," Anal. Biochem., 14:328-336 (1966).
Katre et al., "Chemical Modification of Recombinant Interleukin 2 by Polyethylene Glycol Increases its Potency in the Murine Meth A Sarcoma Model," Proc. Nat'l. Acad. Sci. USA, 84:1487-1491 (1987).
Kodera et al., Seikagaku, 60(9):1005-1018 (1988) "Frontier of Chemical Modification of Proteins: Application of Polyethyleneglycol-modified Enzymes in Biotechnical Processes".
Koerholz et al., "Chemical and Immunological Characteristics of Four Different L-Asparagina Preparations," Eur. J. Haematol., 42(5):417-424 (1982).
Laemmli, "Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4," Nature, 227:680-685 (1970).
Ralph et al., "Inducible Production of Human Macrophage Growth Factor, CSF-1," Blood, 66:633-639 (1988).
Stocks et al., "A Fluorometric Assay of the Degree of Modification of Protein Primary Amines with Polyethylene Glycol," Anal. Biochem., 154:232-234 (1986).
Welte et al., "Purification and Biochemical Characterization of Human Pluripotent Hematopoietic Colony-stimulating Factor," Proc. Nat'l. Acad. Sci. (USA), 82:1526-1530 (1985).
Tanaka et al. "Pharmacokinetics of Recombinant Human Granulocyte Colony-stimulating Factor Conjugated to Polyethylene Glycol in Rats". Cancer Research 51(14):3710-3714, Jul. 1991.
Ishikawa Rika
Kakitani Makoto
Okada Yuji
Kirin-Amgen Inc.
Ulm John
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