Chemically defined medium for human mesenchymal stem cells

Details Chemically defined medium for human mesenchymal stem cells Chemically defined medium for human mesenchymal stem cells

1995-06-05

1999-06-01

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Primate cell, per se

424 937, 435371, 435383, 435384, 435388, 435389, 435404, 435405, 435407, 435372, C12N 500, C12N 508, A61K 3512

Patent

active

059087820

ABSTRACT:
A composition and method for maintaining the viability of human mesenchymal precursor cells in a serum-free environment which composition includes (1) a minimum essential medium; (2) serum albumin; (3) an iron source; (4) insulin or an insulin-like growth factor; and (5) at least one amino acid selected from the group consisting of glutamine, arginine and and cysteine, and is free of serum. Also, a composition and method for culture expanding human mesenchymal precursor cells in a serum-free environment. This composition further includes a mitogen, paricularly a serotonergic agonist. The cells are preferably isolated human mesenchymal stem cells.

REFERENCES:
patent: 4444778 (1984-04-01), Coughlin
patent: 5045467 (1991-09-01), Bertheussen
patent: 5197985 (1993-03-01), Caplan et al.
patent: 5486359 (1996-01-01), Caplan et al.
Seuwen et al., Serotonin stimulates DNA synthesis in fibroblasts acting through 5-HT receptors coupled to G-protein, Nature 335, pp. 254-256. Sep. 1988.
Brower et al. Growth of Cell Lines and Clinical Specimens of Human Non-Small Cell Lung Cancer in a Serum-Free Defined Medium, Cancer Res. 46:798-806. 1986.
Jessop et al. The differentiation of a potential mesenchymal stem cell population within ovine bone marrow. Biochem Soc Transactions 22:248S. 1994.
Maurer. Towards Chemically-defined, serum-free media for mammalian cell culture. In: Animal cell culture a practrical approach (Freshney, ed.) pp. 13-19. (1986).

Affiliated with

Also associated with

Rating

Chemically defined medium for human mesenchymal stem cells does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Chemically defined medium for human mesenchymal stem cells, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chemically defined medium for human mesenchymal stem cells will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-954674