Chemically defined growth medium

Details Chemically defined growth medium Chemically defined growth medium

1987-12-21

1989-07-25

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352403, 43524054, C12N 500

Patent

active

048513466

ABSTRACT:
A growth medium suitable for increasing the yield of plasminogen activator obtained from plasminogen activator-producing cells. The growth medium is composed of a 1:1:1 (by weight) mixture of Waymouth's MB752/1, Dulbecco MEM and Ham's F-12. The growth medium is preferably supplemented with growth factors.

REFERENCES:
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patent: 4560655 (1985-12-01), Baker
Chan, S. Y. et al., Prostate, 2(3): 291-298 (1981), cited in Chem. Abst. CA 99(15): 118810m.
Barka, T. et al., Am. J. Anat., 165(2): 187-198 (1982), cited in Biosis Abst. 83: 216151.
D. C. Rijken, Purification and Characterization of the Plasminogen Activator Secreted by Human Melanoma Cells in Culture, J. Biol. Chem., 256, 7035-7041 (1981).
K. L. Rosenthal, et al., Fibrinolytic Activity Associated with Cultured Human Neoplastic and Normal Cells, Molecular & Cellular Biochemistry, vol. 15, No. 2, pp. 149-153 (1977).
M. E. Kaighn et al., Growth Control of Prostatic Carcinoma Cells in Serum-Free Media: Interrelationship of Hormone Response, Cell Density, and Nutrient Media, Proc. Natl. Acad. Sci., vol. 78, No. 9, pp. 5673-5676 (1981).
L. Ossowski et al., Loss of Malignancy During Serial Passage of Human Carcinoma in Culture and Discordance Between Malignancy and Transformation Parameters, Cancer Research, 40, pp. 2310-2315 (1980).
D. Barnes, et al., Growth of a Human Mammary Tumour Cell Line in a Serum-Free Medium, Nature, Oct., 1979.

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