Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Culture medium – per se
Patent
1995-03-28
1998-01-27
Rollins, John W.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Culture medium, per se
435404, 435366, 435371, 435384, 435325, C12N 500
Patent
active
057121630
ABSTRACT:
The present invention provides systems, methods and chemically defined media for the cultivation of cells, particularly epithelial cells. Cells may be cultured with a varied calcium concentration. Furthermore, a calcium concentation in excess of 1.00 mM may be used in the practice of the present invention without loss of a proliferative cell population and maintianing high colony forming efficiencies. Population doubling times range from about 16 to about 33 hours. Cells may serially cultivated to achieve from about 20 to about 50 population doublings.
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Johnson Eric William
Maresh John Gregory
Meunier Susan Frances
Parenteau Nancy Louise
Buckley Linda M.
Conlin David G.
Organogenesis Inc.
Rollins John W.
Tate Christopher R.
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