Chemical separation and plural-point, surface-enhanced raman...

Chemistry: analytical and immunological testing – Optical result – With reagent in absorbent or bibulous substrate

Reexamination Certificate

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C436S164000, C436S166000, C436S161000

Reexamination Certificate

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06943032

ABSTRACT:
Sol-gel beds and deposits are utilized for SERS analysis of liquid analytes. Measurements are made at multiple points along the length of a column or channel to increase, very significantly, the speed of analysis, and use of the same medium to both separate the chemicals and also for SERS greatly reduces the complexity of such apparatus and enhances the efficiency of the method.

REFERENCES:
patent: 5308495 (1994-05-01), Avnir et al.
patent: 5693152 (1997-12-01), Carron
patent: WO 200133189 (2001-05-01), None
Cabalin et al. “Fast spatially resolved surface-enhanced Raman spectrometry on a silver coated filter paper using charge-coupled device detection”, Anal. Chim. Acta, 1995, v. 310, pp. 337-345.
Li et al. “Silver-coated capillary tube for surface-enhanced Raman scattering”, Vibrational Spectr., 1992, v. 3, pp. 115-120.
Soper et al. “Surface-enhanced resonance Raman spectroscopy of liquid chromatographic analytes on thin-layer chromatographic plates”, Anal. Chem., 1990, v. 62, pp. 1436-1444.
Jang et al. “Photochemical desorption of 4-vinylbenzoic acid adsorbed on silver colloid surfaces”, J. Phys. Chem. B. 1997, v. 101, pp. 1649-1654.
Walker, III et al. “Isotachophoretic separation on a microchip. Normal Raman spectroscopy detection”, Anal. Chem., 1998, vol. 70, pp. 3766-3769.
He et al. “Surface-enhanced Raman scattering: a structure-specific detection method for capillary electrophoresis”; Anal. Chem 2000, v. 72, pp. 5348-5355.
“On-Column Surface-Enhanced Raman Spectroscopy Detection in Capillary Electrophoresis Using Running Buffers Containing Silver Colloidal Sollutions” William F. Nirode, Gerald L. Devault, and Michael J. Sepaniak (Anal. Chem. 2000, 72, 1866-1871), see epecially p. 1867 col. 2, line 9 and Figures 3 and 4.
Molecular-Specific Chromatographic Detector Using Modified SERS Substrates, Keith T. Carron and Brian Kennedy (Anal. Chem. 1995, 67, 3353-3356); see especially Figures 1 and 3.
“Determination of Purine Bases by Reversed-Phase High-Performance Liquid Chromatography Using Real-Time Surface-Enhanced Raman Spectroscopy”, Rongsheng, Fan ni, and Therese M. Cotton (Anal. Chem. 1991, 63, 437-442); see especially Figures 1 and 6.
“Direct Analysis of High-Performance Thick Layer-Chromatography Spots of Nucleic Purine Derivatives by Surface-Enhanced Raman Scattering Spectrometry” Shuichi Kawasaki, Totaro Imasaka, and Nobuhiko Ishibashi (Anal. Chem. 1987, 59, 525-527).

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