Chemical promotion of nucleic acid hybridization

Details Chemical promotion of nucleic acid hybridization Chemical promotion of nucleic acid hybridization

1995-06-13

1998-12-29

Houtteman, Scott W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C12Q 168

Patent

active

058539867

ABSTRACT:
An improved method is described for increasing the rate and specificity of hybridization of target polynucleotide sequences with complementary probes. The method uses amphipathic hydrocarbon polymers (AHP), exemplified by polyvinyl sulfonic acid or polystyrene sulfonic acid, to effect the improved hybridization characteristics. The method may be used in solution phase, solid phase, or in situ hybridization formats.

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