Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-06-15
1998-12-08
Martinell, James
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, C12Q 168, C12P 1934
Patent
active
058467092
ABSTRACT:
The present invention is directed to a method of amplifying and detecting single or double stranded target nucleic acid molecules. Amplification of the target nucleic acid molecule is accomplished by using at least two chemically modified oligonucleotide probes per target nucleic acid molecule to form a joined oligonucleotide product. Each oligonucleotide probe is comprised of a long and short sequence. The long sequence of each probe hybridizes to adjacent regions of the target nucleic acid molecule. The short sequences of each probe hybridize to each other. Chemical functionality groups attached to the short sequences of each oligonucleotide probe covalently combine linking the probes to form a joined oligonucleotide product. The joined oligonucleotide product is formed without the use of enzymes.
The reactivity of the chemical functionality groups on each probe is target dependent. The chemical functionality group on each probe is prevented from reacting with other chemical functionality groups on other probes unless the probes are properly hybridized to the target molecule and to each other, as described above. The chemical functionality groups are covalently attached to the short sequence of each probe in such a way that they are sheltered or protected from the chemical functionality groups of other probes while the probes are in solution. Only when the short sequences of adjacent probes are hybridized to each other are the chemical functionality groups on the probes brought into close enough proximity to form a covalent bond and join the probes to form a joined oligonucleotide product.
REFERENCES:
patent: 4638195 (1987-01-01), Mullis et al.
patent: 5185243 (1993-02-01), Ullman et al.
Feit Irving N.
Gallagher Thomas C.
ImClone Systems Incorporated
Martinell James
Sheets Eric J.
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