Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1996-01-16
1998-03-17
Elliott, George C.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 231, 536 241, 536 245, 536 251, C07H 2100, C07H 2102, C07H 2104
Patent
active
057288186
ABSTRACT:
Method for synthesis of an enzymatic nucleic acid by providing a 3' and a 5' portion of the enzymatic nucleic acid having independent chemically reactive groups at the 5' and 3' positions, respectively, under conditions in which a covalent bond is formed between the 3' and 5' portions by the chemically reactive groups. The bond is selected from the group consisting of, disulfide, morpholino, amide, ether, thioether, amine, a double bond, sulfonamide, ester, carbonate, and hydrazone. The bond is not the natural bond formed between a 5' phosphate group and a 3' hydroxyl group.
REFERENCES:
patent: 5298612 (1994-03-01), Jennings et al.
patent: 5610054 (1997-03-01), Draper et al.
Moore et al "Site Specific Modification of Pre-mRNA; The 2'-Hydroxyl Groups at the Spcice Sites", Science vol. 256:992-997, 1992.
Castanotto et al "Biological and Functional Aspects of Latalytic DNAs", Critical Reviews Eukaryotic Gene Expression, vol. 2(4):331-357, 1992.
Chowrira et al "Extensive Phosphorothioate Substitution Yields Highly Active and Nuclease Resistant Hairpin ribozymes". NAR vol. 20(11):2835-2840, 1992.
McCall et al "Minimal Sequence Requirements for Ribozyme Actiuim", DNAs vol. 89:5710-5714, 1992.
Hendry et al "Using Linkers to Investigate the Spatial Separation of the conserved Nucleotides"A.sub.9 and G.sub.12 in the Hammer Head Ribozyme. Biochimia Biophysica ACTA vol. 1219:405-412, 1994.
Cech, "Ribozymes and Their Medical Implications," JAMA 260:3030-3034 (1988).
Haseloff and Gerlach, "Simple RNA Enzymes with New and Highly Specific Endoribonuclease Activities," Nature 334:585-591 (1988).
Hui, "Synthesis and Properties of Borohydride Derivatives," Inorg. Chem. 19:3185-3186 (1980).
Jeffries and Symons, "A Catalytic 13-mer Ribozyme," Nucleic Acids Research 17:1371-1377 (1989).
Kim and Cech, "Three-dimensional model of the active site of the self-splicing rRNA precursor of Tetrahymena,"Proc. Natl. Acad. Sci. USA 84:8788-8792 (1987).
Scaringe et al., "Chemical synthesis of biologically active oligoribonucleotides using .beta.-cyanoethyl protected ribonucleoside phosphoramidites," Nucl Acids Res. 18:5433-5441 (1990).
Uhlenbeck, "A Small Catalytic Oligoribonucleotide," Nature 328:596-600 (1987).
Usman et al., "Automated Chemical Synthesis of Long Oligoribonucleotides Using 2'-O-Silylated Ribonucleoside 3'-O-Phosphoramidites on a Controlled-Pore Glass Support: Synthesis of a 43-Nucleotide Sequence Similar to the 3'-Half Molecule of an Escherichia coli Formylmethoionine tRNA," J. Am. Chem. Soc. 109:7845-7854 (1987).
Wincott et al., "Synthesis, deprotection, analysis and purification of RNA and ribozymes," Nucleic Acids Research 23:2677-2684 (1995).
Usman Nassim
Wincott Francine
Elliott George C.
McGarry Sean
Ribozyme Pharmaceuticals Inc.
LandOfFree
Chemical linkage of ribozyme protions does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Chemical linkage of ribozyme protions, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chemical linkage of ribozyme protions will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-958646