Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...
Reexamination Certificate
2007-06-12
2007-06-12
Kubelik, Anne (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
C800S295000, C800S288000, C536S023100, C536S023510, C536S024100, C435S320100, C435S468000
Reexamination Certificate
active
10129849
ABSTRACT:
Disclosed is a chemically inducible promoter for transforming plants or plant cells with genes which are regulatable by adding the plants or cells to a medium containing an inducer or by removing them from such medium. The promoter is inducible by a glucocorticoid, estrogen or inducer not endogenous to plants. Such promoters may be used with any plant genes that can promote shoot regeneration and development to induce shoot formation in the presence of a glucocorticoid, estrogen or inducer. The promoter may be used with antibiotic or herbicide resistance genes or other genes which are regulatable by the presence or absence of a given inducer. Also presented are organisms or cells comprising a gene wherein the natural promoter of the gene is disrupted and the gene is placed under the control of a transgenic inducible promoter. These organisms and cells and their progeny are useful for screening for conditional gain of function and loss of function mutations.
REFERENCES:
patent: 5880331 (1999-03-01), Krebbers et al.
patent: 5942662 (1999-08-01), Ryals et al.
patent: 6031151 (2000-02-01), Draper
patent: 6147282 (2000-11-01), Goff et al.
patent: 334 383 (1989-09-01), None
patent: 334 383 (1989-09-01), None
patent: WO 93/07272 (1993-04-01), None
patent: WO 98/05754 (1998-02-01), None
patent: WO 98/05754 (1998-02-01), None
patent: WO 98/36637 (1998-08-01), None
patent: WO 99/38988 (1999-08-01), None
patent: WO 99/38988 (1999-08-01), None
patent: WO 99/38988 (1999-08-01), None
Zuo et al The Plant Journal 2000 24:265-273 p. 271.
Akiyoshi, D.E. et al. “T-DNA ofAgrobacterium tumefaciensencodes an enzyme of cytokinin biosynthesis”,Proc. Natl. Acad. Sci. USA, Oct. 1984; 81:5994-5998.
Aoyama, T. and Chua, N.-H. “A glucocorticoid-mediated transcriptional induction system in transgenic plants”,Plant J.(1997); 11(3):605-612.
Aoyama, T. et al. “Ectopic Expression of the Arabidopsis Transcriptional Activator Athb-1 Alters Leaf Cell Fate in Tobacco”,Plant Cell, Nov. 1995; 7:1773-1785.
Aoyama T. “Glucocorticoid-inducible Gene Expression in Plants”,Inducible Gene Expression in Plants(Ed. P. Reynolds), CAB International (Wallingford) (1999), pp. 43-59.
Barry, G.F. et al. “Identification of a cloned cytokinin biosynthetic gene”,Proc. Natl. Acad. Sci. USA, Aug. 1984; 81:4776-4780.
Beato, M. “Gene Regulation by Steroid Hormones”,Cell, Feb. 10, 1989; 56:335-344.
Becker, D. et al. “New plant binary vectors with selectable markers located proximal to the left T-DNA border”Plant Molecular Biology(1992); 20:1195-1197.
Benfey, P.N. and Chua, N.-H. “The Cauliflower Mosaic Virus 35S Promoter: Combinatorial Regulation of Transcription in Plants”,Science, Nov. 16, 1990; 250:959-966.
Bevan, M.W. and Chilton, M.-D. “T-DNA of the Agrobacterium T1 and RI Plasmids”,Ann. Rev. Genet., 1982; 16:357-384.
Boase, M.R. et al. Genetic Transformation Mediated byAgrobacterium tumefaciensof Florists′ Chrysanthemum (Dendranthema xgrandiflorum) Cultivar ‘Peach Margaret’,In Vitro Cell. Dev. Biol.-Plant, Mar. 1998; 34:46-51.
Böhner, S. et al. “Transcriptional activator TGV mediates dexamethasone-inducible and tetracycline-inactivatable gene expression”,The Plant Journal(1999); 19(1):87-95.
Braselmann, S. et al. “A selective transcriptional induction system for mammalian cells based on Ga14-estrogen receptor fusion proteins”,Proc. Natl. Acad. Sci. USA, Mar. 1993; 90:1657-1661.
Bruce, W. et al. “Expression Profiling of the Maize Flavonoid Pathway Genes Controlled by Estradiol-inducible Transcription Factors CRC and P,”The Plant Cell(2000) 12:65-79.
Chuck, G. et al. “KNAT1 Induces Lobed Leaves with Ectopic Meristems When Overexpressed in Arabidopsis”,The Plant Cell, Aug. 1996; 8:1277-1289.
Coruzzi, G. et al. “Tissue-specific and light-regulated expression of a pea nuclear gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase”,The EMBO Journal, 1984; 3(8):1671-1679.
Dalrymple, M.A. et al. “DNA sequence of the herpes simplex virus type 1 gene whose product is responsible for transcriptional activation of immediate early promoters”,Nucleic Acids Research, 1985; 13(21):7865-7879.
De Veylder, L. et al. “Herbicide Safener-Inducible Gene Expression inArabidopsis thaliana”, Plant Cell Physiol., 1997; 38(5):568-577.
Ebinuma, H. et al. “Selection of marker-free transgenic plants using the isopentenyl transferase gene”,Proc. Natl. Acad. Sci. USA, Mar. 1997; 94:2117-2121 + cover page.
Faiss, M. et al. “Conditional transgenic expression of theiptgene indicates a function for cytokinins in paracrine signaling in whole tobacco plants”,The Plant Journal, 1997; 12(2):401-415.
Gan, S. and Amasino, R.M. “Inhibition of Leaf Senescence by Autoregulated Production for Cytokinin”,Science, Dec. 22, 1995; 270:1986-1988.
Gatz, C. “Chemically inducible promoters in transgenic plants”,Curr. Opin. Biotechnol., 1996; 7:168-172.
Gatz, C. et al. “Stringent repression and homogeneous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants”,The Plant Journal, 1992; 2(3):397-404.
Gatz, C. “Chemical Control of Gene Expression”,Annu. Rev. Plant Physiol. Plant Mol. Biol., 1997; 48:89-108.
Gatz, C. and Lenk, I. “Promoters that respond to chemical inducers”,Trends Plant Sci., Sep. 1998; 3(9):352-358.
Giniger, E. et al. “Specific DNA Binding of GAL4, A Positive Regulatory Protein of Yeast”,Cell, Apr. 1985; 40:767-774.
Goodrich, J.A. et al. “Drosophila TAFII40 Interacts with Both a VP16 Activation Domain and the Basal Transcription Factor TFIIB”,Cell, Nov. 5, 1993; 75:519-530.
Greene, G.L. et al. “Sequence and Expression of Human Estrogen Receptor Complementary DNA”,Science, Mar. 1986; 231:1150-1154.
Harms, C.T. et al. “Herbicide resistance due to amplification of a mutant acetohydroxyacid synthase gene”,Mol. Gen. Genet., 1992; 233:427-435.
Hattori, J. et al. “Multiple resistance to sulfonylureas and imidazolinones conferred by an acetohydroxyacid synthase gene with separate mutations for selective resistance”,Mol. Gen. Genet., 1992; 232:167-173.
Hattori, J. et al. “An acetohydroxy acid synthase mutant reveals a single site involved in multiple herbicide resistance”,Mol. Gen. Genet., 1995; 246:419-425.
Horii, T. et al. “Nucleotide Sequence of thelexAGene ofE. coli”, Cell, Mar. 1981; 23:689-697.
Ishige, F. et al. “A G-box motif (GCCACGTGCC) tetramer confers high-level constitutive expression in dicot and monocot plants”,The Plant Journal, 1999; 18(4):443-448.
Kakimoto, T. “CKl1, a Histidine Kinase Homolog Implicated in Cytokinin Signal Transduction”,Science, Nov. 8, 1996; 274:982-985.
Kang, H-G et al. “A glucocorticoid-inducible transcription system causes severe growth defects inArabidopsisand induces defense-related genes”,The Plant Journal, 1999; 20(1):127-133.
Keegan, L. et al. “Separation of DNA Binding from the Transcription-Activating Function of a Eukaryotic Regulatory Protein”,Science, Feb. 1986, 231:699-704.
Klee, H. et al. “Agrobacterium-Mediated Plant Transformation and its Further Applications to Plant Biology”,Ann. Rev. Plant Physiol., 1987; 38:467-486.
Klee, H. et al. “E2. Transgenic Plants in Hormone Biology”, inPlant Hormones(ed. P.J. Davies) (Kluwer Academic Publishers (Netherlands)) (1995), pp. 340-353.
Kost, B. et al. “A GFP-mouse talin fusion protein labels plant actin filaments in vivo and visualizes the actin cytoskeleton in growing pollen tubes”,The Plant Journal, 1998; 16(3):393-401.
Kunkel, T. et al. “Inducible isopentenyl transferase as a high-efficiency marker for plant transformation”,Nature Biotechnology, Sep. 1999; 17:916-919.
Laughon, A
Chua Nam-Hai
Zuo Jianru
Kubelik Anne
Page Brent T
Rothwell Figg Ernst & Manbeck P.C.
The Rockefeller University
LandOfFree
Chemical inducible promoter used to obtain transgenic plants... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Chemical inducible promoter used to obtain transgenic plants..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Chemical inducible promoter used to obtain transgenic plants... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3808997