Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-03-10
1996-11-05
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
4351885, 435 41, C12Q 125, C12N 900
Patent
active
055716814
ABSTRACT:
Molecules having covalent catalytic activity are identified by panning synthetic and semisynthetic combinatorial libraries on solid phase suicide substrates. In an alternative mode, mechanism-based inhibitors or affinity labels may substituted for the suicide substrate. Covalent catalysts within the combinatorial library form covalent conjugates with the suicide substrate, mechanism-based inhibitor, or affinity label. Covalent conjugates are immobilized by attachment to the suicide substrate to solid phase and are easily separated from unconjugated elements of the combinatorial library by stringent washing. Combinatorial libraries employing phagemid-display are particularly preferred since such phagemids include genetic material for identifying and amplifying conjugated catalysts. Covalent catalysts obtainable by this method include, inter alia, molecules having esterolytic activity, aldol condensation activity, .beta.-lactamase activity, glycosidase activity, RNase activity, and proteolytic activity.
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Lerner, et. al. "At The Crossroads Of Chemistry And Immunology: Catalytic Antibodies" Science, 252: 659-667 (1991).
Lerner, et. al. "Semisynthestic Combinatorial Antibody Libraries: A Chemical Solution To The Diversity Problem", Proc. Natl. Acad. Sci., 89: 4457-4461 (1992).
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Lewis Donald G.
Patterson Jr. Charles L.
The Scripps Research Institute
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