Chemical blocking agent against non-specific binding or staining

Details Chemical blocking agent against non-specific binding or staining Chemical blocking agent against non-specific binding or staining

1986-09-15

1989-04-04

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435810, 436503, 436519, 436548, 436825, G01N 5300, G01N 53554

Patent

active

048186864

ABSTRACT:
A chemical agent is provided for significantly preventing or blocking non-specific staining or binding of an antibody specific for Terminal deoxynucleotidyl Transferase (TdT) during immunofluorescent or immunoperoxidase assay procedures. These procedures include both immunofluorescent and immunohistochemical staining of samples followed by flow cytometric and/or microscopic analysis, respectively. The invention is practiced by selective use of casein introduced into the assay procedures at an appropriate interval prior to analysis using a labelled or tagged monoclonal antibody specific to a TdT epitope. The casein utilized successfully was obtained from a large variety of sources and includes the use of a non-fat milk product.

REFERENCES:
patent: 3988115 (1976-10-01), Modabber
patent: 4307189 (1981-12-01), Kit
patent: 4460559 (1984-07-01), Goldenberg
patent: 4515890 (1985-05-01), Manderino et al.
patent: 4582791 (1986-04-01), Khanna et al.
patent: 4680274 (1987-07-01), Sakai et al.

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