Chemistry: electrical and wave energy – Processes and products – Processes of treating materials by wave energy
Patent
1989-09-25
1992-08-25
Niebling, John F.
Chemistry: electrical and wave energy
Processes and products
Processes of treating materials by wave energy
20415793, 534564, C07C24508, C07C 4500
Patent
active
051416271
ABSTRACT:
The present invention relates to a group of organic chelators whose affinity for calcium ion in solution is increased by electromagnetic radiation. Specifically, the chelators are related to BAPTA and utilize the addition of an electron-withdrawing group (e.g., diazocarbonyl) to a ring of BAPTA, para to the amino group. Photochemical rearrangement of the diazoacetyl group converts the group to the electron-donating carboxymethyl group, causing the calcium ion efficiency to increase 25 to 50 fold. These chelators when incorporated into rat fibroblasts either by microinjection or by incubation as the membrane-permeable, enzymatically-labile tetraacetoxymethyl ester and flash-photolyzed cause a drop in intracellular free calcine ion to or below resting valves of about 10.sup.-7 M. These chelators are used to generate controlled fast removal of intracellular free calcium ion to mimic or modulate a number of important cellular responses, especially in nerve or muscle.
REFERENCES:
patent: 4603209 (1986-07-01), Tsien
patent: 4689432 (1987-08-01), Tsien
patent: 4806604 (1989-08-01), Tsien
I. P. Mulligan & C. C. Ashley, "Rapid Relaxation of Single Frog Skeletal Muscle Fibres Following Laser Flash Photolysis of the Caged Calcium Chelator, Diazo-2", Federation of European Biochemical Societies, 1989, vol. 255, p. 196.
S. R. Adams, J. P. Y. Kao & R. Y. Tsien, "Biologically Useful Chelators That Take Up Ca.sup.2+ Upon Illumination,", Journal of the American Chemical Society, 1989, vol. 111, pp. 7957-7968.
S. R. Adams, et al., "Biologically Useful Chelators That Release Ca.sup.2+ Upon Illumination," Journal of the American Chemical Society, 1988, vol. 110, pp. 3212-3220.
Grzegorz Grynkiewicz, et al., "A New Generation of C.sup.2+ Indicators with Greatly Improved Fluorescence Properties," The Journal of Biological Chemistry, 1985, vol. 260, pp. 3440-3450.
James A. McCray, "Properties and Uses of Photoreactive Caged Compounds," Annu. Rev. Biophys. Biophys. Chem., 1989, vol. 18, pp. 239-270.
Alison M. Gurney & Henry A. Lester, "Light-Flash Physiology With Synthetic Photosensitive Compounds," Physiological Reviews 1987, vol. 67, pp. 583-617.
Akwasi Minta, et al., "Fluorescent Indicators for Cytosolic Calcium Based on Rhodamine and Fluorescein Chromophores," The Journal of Biological Chemistry, 1989, vol. 264, pp. 8171-8178.
Adams Stephen R.
Tsien Roger Y.
Marquis Steven P.
Niebling John F.
The Regents of the University of California
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