Charge effects in enzyme immunoassays

Details Charge effects in enzyme immunoassays Charge effects in enzyme immunoassays

1979-07-26

1981-09-01

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 7, 435188, 435810, 424 12, 23230B, C12Q 170, G01N 3354, C12N 996

Patent

active

042873002

ABSTRACT:
A method for determining a member of a specific binding pair-ligand and receptor (antiligand). Reagents employed include a first modified member which provides an electrical field due to the presence of a plurality of ionic charges and a second modified member labeled with a component of a signal producing system, which system may have one or more components. The average proximity in the assay medium of the first and second modified members is related to the amount of analyte, where the observed signal from the signal producing system is related to the effect of the electrical field on the signal producing system.
Also, compositions are provided, as well as reagents, in predetermined ratios for optimizing the signal response to variations in analyte concentration.

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