Charcoal stabilization of phenyl phosphates

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

Reexamination Certificate

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C435S021000, C435S183000, C436S008000, C436S145000, C436S146000, C436S176000

Reexamination Certificate

active

11262715

ABSTRACT:
The invention discloses methods, compositions and kits for stabilizing a solubilized phenyl phosphate, preferably paranitrophenyl phosphate (PNPP), using charcoal. Also disclosed are methods, compositions and kits for recycling solubilized phenyl phosphate, preferably PNPP, that has an absorbance of less than 0.1 when measured at 405 nm due to non-enzymatic hydrolysis.

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HowStuffWorks, “What is activated charcoal and why is it used in filters?” http://www.howstuffworks.com/question 209.htm accessed Nov. 3, 2004.
Sigma-Aldrich Product Catalogue, “2-Amino-2-methyl-1,3-propanediol” (AMPD), 2004, http://www.sigmaaldrich.com/cgi-bin/hsrun/Suite7/Suite/Suite.hjx;start=Suite.HsViewHierarchy.run?Detail=Product&ProductNumber=SIGMA-A5888&VersionsSequence1, accessed Nov. 3, 2004.
Sigma-Aldrich Product Catalogue, “BIS-TRIS”2004, http://www.sigmaaldrich.com/cgi-bin-hsrun/Suite7/Suite/Suite.hjx;start=Suite.HsViewHierarchy.run?Detail=Product&ProductNumber=SIGMA-B7535&VersionsSequence1, accessed Nov. 3, 2004.

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