Charcoal stabilization of phenyl phosphates

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

Reexamination Certificate

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C435S021000, C435S183000, C436S008000, C436S145000, C436S146000, C436S176000

Reexamination Certificate

active

07001717

ABSTRACT:
The invention discloses methods, compositions and kits for stabilizing a solubilized phenyl phosphate, preferably paranitrophenyl phosphate (PNPP), using charcoal. Also disclosed are methods, compositions and kits for recycling solubilized phenyl phosphate, preferably PNPP, that has an absorbance of less than 0.1 when measured at 405 nm due to non-enzymatic hydrolysis.

REFERENCES:
patent: 1958210 (1934-05-01), Scott
patent: 3951859 (1976-04-01), Inaba et al.
patent: 4028465 (1977-06-01), Lewin et al.
patent: 4029593 (1977-06-01), Schäpel et al.
patent: 4042335 (1977-08-01), Clément
patent: 4132598 (1979-01-01), Modrovich
patent: 4157280 (1979-06-01), Halbert et al.
patent: 4372874 (1983-02-01), Modrovich
patent: 4594249 (1986-06-01), Procter et al.
patent: 5464560 (1995-11-01), Schilling et al.
patent: 5895819 (1999-04-01), Hagi
patent: 5948631 (1999-09-01), Nagel et al.
patent: 2002/0058279 (2002-05-01), Fritsch et al.
Siegenthaler, E “Phosphatase test with sodium p-nitrophenyl phosphate” Mitteilungen asu dem Gebiete der Lebensmitteluntersuchung und Hygiene, 1959, vol. 47, translation provided.
HowStuffWorks, “What is activated charcoal and why is it used in filters?” http://www.howstuffworks.com/question 209.htm accessed Nov. 3, 2004.
Sigma-Aldrich Product Catalog, “2-Amino-2-methyl-1,3-propanediol” (AMPD), 2004, http://www.sigmaaldrich.com/cgi-bin/hsrun/Suite7/Suite/Suite.hjx;start=Suite.HsViewHierarchy.run?Detail=Product&ProductNumber=SIGMA-A5888&VersionsSequence1, accessed Nov. 3, 2004.
Sigma-Aldrich Product Catalog, “BIS-TRIS”2004, http://www.sigmaaldrich.com/cgi-bin-hsrun/Suite7/Suite/Suite.hjx;start=Suite.HsViewHierarchy.run?Detail=Product&ProductNumber=SIGMA-B7535&VersionsSequence1, accessed Nov. 3, 2004.
Bowers, George N. Jr. and Robert B. McComb, “Measurement of Total Alkaline Phosphatase Activity in Human Serum”, Clinical Chemistry, 1975, vol. 21, No. 13, pp. 1988-1995.
International Search Report dated Feb. 24, 2005.

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