Characterizing polypeptides through cleavage and mass...

Details Characterizing polypeptides through cleavage and mass... Characterizing polypeptides through cleavage and mass...

2005-01-25

2005-01-25

Weber, Jon P. (Department: 1653)

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

Amino acid or sequencing procedure

C436S086000, C436S173000, C435S023000, C435S024000

Reexamination Certificate

active

06846679

ABSTRACT:
Provided is a method for characterizing a polypeptide or a population of polypeptides, which method comprises: (a) contacting a sample comprising one or more polypeptides with a first cleavage agent to generate polypeptide fragments; (b) isolating one or more polypeptide fragments, each fragment comprising the N-terminus or the C-terminus of the polypeptide from which it was fragmented; (c) identifying the isolated fragments by mass spectrometry; (d) repeating steps (a)-(c) on the sample using a second cleavage agent that cleaves at a difference site from the first cleavage agent; and (e) characterizing the one or more polypeptides in the sample from the fragments identified in steps (c) and (d).

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