Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1982-10-01
1985-08-13
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 15, 435 18, 435 91, 4351723, 436504, 436515, 436 63, 436 64, 436 94, 436813, 536 27, 424 11, 935 77, 935 78, C12Q 168, C12Q 148, C12Q 134, C12P 1934, C12N 1500, G01N 1502, C07H 2104, A61K 4300
Patent
active
045350582
ABSTRACT:
Experiments designed to define the differences between an oncogene isolated from human bladder cancer cells and its corresponding proto-oncogene are described herein. By a series of in vitro recombinations, the difference was initially isolated to a 350 kb segment of DNA; sequencing defined the difference as a change in the Gly.sup.12 codon causing the p21 protein of the oncogene to contain valine at a location where the p21 protein of the proto-oncogene contained glycine. Assays for detecting carcinogenesis based on such differences are also described. In one type of assay, a restriction enzyme specific for either the altered or non-altered DNA segment of the genes are employed to detect carcinogenesis. In another type of assay, seralogical reagents, such as antibody specific for either p21 protein expressed from the proto-oncogene or oncogene, or a common site therein, are described.
REFERENCES:
patent: 4299815 (1981-11-01), Hansen et al.
patent: 4368262 (1983-01-01), Bucovaz et al.
patent: 4395486 (1983-07-01), Wilson et al.
Maniatis et al. (ed.): Molecular Cloning--A Laboratory Manual, Cold Spring Harbor Laboratory, 1982, p. 100.
Butler: Gene Control in the Living Cell, Basic Books, 1968, pp. 125-127.
Santos et al.: Nature 298, 343 (1982).
Ellis et al.: J. Virol. 36, 408 (1980).
Courtneidge et al.: Proc. Natl. Acad. Sci. USA 77, 3783 (1980).
Bradley Scott M.
Tabin Clifford J.
Weinberg Robert A.
Brook David E.
Martinell James
Massachusetts Institute of Technology
Smith, Jr. Arthur A.
Wiseman Thomas G.
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