Characterization of individual polymer molecules based on...

Details Characterization of individual polymer molecules based on... Characterization of individual polymer molecules based on...

2007-03-13

2007-03-13

Patterson, Jr., Charles L. (Department: 1652)

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

C435S004000, C436S151000

Reexamination Certificate

active

10739585

ABSTRACT:
The invention relates to a method for detecting a double-stranded region in a nucleic acid by (1) providing two separate, adjacent pools of a medium and a interface between the two pools, the interface having a channel so dimensioned as to allow sequential monomer-by-monomer passage of a single-stranded nucleic acid, but not of a double-stranded nucleic acid, from one pool to the other pool; (2) placing a nucleic acid polymer in one of the two pools; and (3) taking measurements as each of the nucleotide monomers of the single-stranded nucleic acid polymer passes through the channel so as to differentiate between nucleotide monomers that are hybridized to another nucleotide monomer before entering the channel and nucleotide monomers that are not hybridized to another nucleotide monomer before entering the channel.

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