Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Carbohydrate doai
Reexamination Certificate
2006-02-28
2006-02-28
Woitach, Joseph (Department: 1632)
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Carbohydrate doai
C536S023100, C536S024500, C435S006120, C435S325000
Reexamination Certificate
active
07005423
ABSTRACT:
There is provided a method of identifying DNA responsible for conferring a particular phenotype in a cell which method comprises a) constructing a cDNA or genomic library of the DNA of the cell in a suitable vector in an orientation relative to a promoter(s) capable of initiating transcription of the cDNA or DNA to double stranded (ds) RNA upon binding of an appropriate transcription factor to the promoter(s), b) introducing said library into one or more of the cells comprising the transcription factor, and c) identifying and isolating a particular phenotype of the cell comprising the library and identifying the DNA or cDNA fragment from the library responsible for conferring the phenotype. Using this technique it is also possible to assign a function to a known DNA sequence by a) identifying a homologue(s) of the DNA sequence in a cell, b) isolating the relevant DNA homologues(s) or a fragment thereof from the cell, c) cloning the homologue or fragment thereof into an appropriate vector in an orientation relative to a suitable promoter(s) capable of initiating transcription of dsRNA from the DNA homologue or fragment upon binding of an appropriate transcription factor to the promoter(s), and d) introducing the vector into the cell from step a) comprising the transcription factor.
REFERENCES:
patent: 6506559 (2003-01-01), Fire et al.
patent: 2 088 379 (1994-07-01), None
patent: WO 90/14090 (1990-11-01), None
patent: WO 91/15111 (1991-10-01), None
patent: WO 95/34680 (1995-12-01), None
patent: WO 96/38553 (1996-12-01), None
patent: WO 96/38555 (1996-12-01), None
patent: WO 99/32619 (1999-07-01), None
patent: WO 99/53050 (1999-10-01), None
patent: WO 99/61631 (1999-12-01), None
Fire et al., Potent and specific genetic interference by double-starded RNA inCaenorhabditis elegans,Feb. 19, 1998, Nature, vol. 391, pp. 806-811.
Deonarain et al., Ligand-targeted receptor-mediated vectors for gene delivery, 1998, Exp. Opin., Ther. Patents, vol. 8, No. 1 pp. 53-69.
Verma et al., Gene therapy-promises,problems and prospects, Sep. 18, 1997, Nature, vol. 389, pp. 239-242.
Crystal, Transfer of Genes to Humans: Early Lessons and Obstacles to Success, 1995, Science, pp. 404-410.
http://genomics.phrma.org/lexicon/p.html#phenotype.
Vennema et al.,Gene108(2):201-209 (1991).
Gast, F.,Nucleic Acids Res.17(23):10109 (1989).
Fire et al.,Nature391:806-811 (1998).
Bogeart et al., Database Geneseq Acc. No. T71322 (1997) & abstract WO96 38555 (1996).
Chien et al.,Proc Natl Acad Sci USA88:9578-9582 (1991).
James et al., Database Medline Acc. No. 97132579.
James et al., Database EMBL Acc. No. U70025 (1996).
Timmons and Fire,Nature395:854 (1998).
Timmons and Fire,East Coast Worm Meeting Abstract 180(Jun. 27, 2002).
Bogart Thierry
Mortier Katherine
Plaetinck Geert
Platteeuw Christ
Devgen NV
Woitach Joseph
Wolf Greenfield & Sacks P.C.
LandOfFree
Characterization of gene function using double stranded RNA... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Characterization of gene function using double stranded RNA..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Characterization of gene function using double stranded RNA... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3662749