Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1994-01-19
1997-07-01
Fleisher, Mindy
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4352523, 435320, 536 232, C12N 121, C12N 1552, C12N 1563
Patent
active
056437919
ABSTRACT:
A biologically pure DNA sequence which encodes an endo 1,4-.beta.-glucanase, useful for the efficient conversion of cellulose to glucose, is disclosed. Also disclosed are recombinant DNA cloning vehicles (vectors) that contain nucleotide sequences that encode for the aforesaid endoglucanase, proteins that exhibit endoglucanase activity, expression-controlling DNA sequences, microorganisms that contain recombinant DNA cloning vehicles, as well as messenger RNA sequences complementary to a DNA strand of the aforesaid DNA nucleotide sequences.
REFERENCES:
patent: 4666828 (1987-05-01), Gusella
patent: 4703008 (1987-10-01), Lin
Beguin et al., "Sequence of a Cellulase Gene of the Thermophilic Bacterium Clostridium thermocellum", Journal of Bacteriology, 162:(1):102-105 (Apr. 1985).
Bibb et al., "The Relationship Between Base Composition and Condon Usage in Bacterial Genes and It use for the Simple and Reliable Identification of Protein-Coating Sequences", Gene, 30:157-166 (1984).
Chamblis et al., "Baterial in vitro Protein-Synthesizing Systems", Methods in Enzymology, 101:[37]:598-605 (1983).
Collmer and Wilson, "Cloning and Expression of a Thermomonospora YX Endocellulase Gene in E. coli", Bio/Technology, pp. 594-601 (Sep. 1983).
Cornet et al., "Characterization of Two Cel (Cellulose Degradation) Genes of Clostridium thermocellum Coding for Endoglucanases", pp. 589-594 (Sep. 1983).
Curry et al., "Expression and Secretion of a Cellulomonas fimi Exoglucanase in Saccharomyces cerevisiae", Applied and Environmental Microbiology, 54:(2):476-484 (1988).
Gilkes et al., "Isolation and Characterization of Escherichia coli Clones Expressing Cellulase Genes from Cellulomonas fimi", J. Gen. Microbial. 130:1377-1384 (1984).
Gilkes et al., "Mode of Action and Substrate Specificities of Cellulases from Cloned Baterial Genes", J. Biol. Chem., 259:(16):10455-10459 (1984).
Gilkes et al., "A Mutant of Escherichia coli that Leaks Cellulase Activity Encoded by Cloned Cellulase Genes from Cellulomonas fimi", Bio/Technology, pp. 259-263 (Mar. 1984).
Gilkes et al., "Precise Excision of the Cellulose Binding Domains from Two Cellulomonas fimi Cellulases by a Homologous Protease and the Effect on Catalysis", J. Biol. Chem., 263:(21):10401-10407 (1988).
Gilkes et al., "Domains in Microbial .beta.-1,4-Glucanases; Sequence Conversation, Function, and Enzyme Families", Microbiological Reviews, 55:(2):303-315 (Jun. 1991).
Guo and Wu, "Exonuclease III: Use for DNA Sequence Analysis and in Specific Deletions of Nucleotides," Methods in Enzymology, 100:60-96 (1983).
Hinchliffe and Box, "Expression of the Cloned Endo-1,3-1,4-.beta.-glucanase Gene of Bacillus subtilis in Saccharomyces cerevisiae", Current Genetics, 8:471-475 (1984).
Inouye and Halegoua, "Secretion and Membrane Localization of Proteins in Escherichia coli", CRC Critical Reviews in Biochemistry, 7:339-371 (1980).
Kilburne et al., "Characterization of the Cellulases of Cellulomonas fimi and cloning of their Structural Genes in Escherichia coli", Abstr.Pap.Am.Chem.Soc. 189 Meet., Cell 55 (1985); provided as: Derwent Biotechnology Abstracts, 4:(2):85-12334 (1985).
Kotoujansky et al., "Molecular Cloning of Erwinia chrysanthemi Pectinase and Cellulase Structural Genes," The EMBO Journal, 4:(3):781-785 (1985).
Kreil, "Transfer of Proteins Across Membranes", Ann. Rev. Biochem., 50:317-48 (1981).
Lansford et al., "The Cellulase System of Cellulomonas fimi", Journal of General Microbiology, 130:11367-1376 (1984).
Lansford et al., "Glycosylation of Bacterial Cellulases Prevents Proteolytic Cleavage Between Functional Domains", FEBS Letters, 225:(1, 2):163-167 (1987).
Maniatis et al., "Enzmes Used in Molecular Cloning", Molecular Cloning, A Laboratory Manual, pp. 100-101 (1982).
Messing, "New M13 Vectors for Cloning", Methods in Enzymology, 101:20-78 (1983).
Miller et al., "Measurement of Carboxymethylcellulae Activity", Analytical Biochemistry, 2:127-132 (1960).
Millet et al., "Cloning of Ten Distinct DNA Fragments of Clostridium thermocellum Coding for Cellulases", FEMS Letters, 29:145-149 (1985).
Moser et al., "Purification and Characterization of Endoglucanase C of Cellulomona fimi, Cloning of the Gene, and Analysis of In Vivo Transcripts of the Gene", Applied and Environmental Microbiology, 55:(10):2480-2487 (Oct. 1989).
Murphy et al., "The DNA Sequence of the Gene and Genetic Control Sites for the Excreted B. subtilis Enzyme", Nucleic Acids Research, 12:(13):5355-5367 (1984).
Ohmura et al., "Length and Structural Effect of Signal Peptides Derived from Bacillus subtilis .alpha.-amylase on secretion of Escherichia coli .beta.-lactamase in B. subtilis cells", Nucleic Acids Research, 12:(13):5307-5318 (1984).
O'Neill et al., "Structural of the Gene Encoding the Exoglucanase of Cellulomonas fimi", Gene, 44:325-330 (1986).
Owolabi et al., "Expression in Escherichia coli of Cellulomonas fimi Structural Gene for Endoglucanase B", Applied and Environmental Microbiology, 54:(2):518-523 (1988).
Sancar et al., "Simple Method for Identification of Plasmid-Coded Proteins", Journal of Bacteriology, 137:(1):692-693 (1979).
Shoemaker et al., "Molecular Cloning of Exo-Cellobiohydrolase I Derived from Trichoderma reeseistrain L27", Bio/Technology, pp. 691-696 (Oct. 1983).
Skipper et al., "Secretion of a Bacterial Cellulase by Yeast", Science, 230:958-960 (1985).
Stuber and Bujard, "Organization of Transcriptional Signals in Plasmids pBR322 and pACYC184", Proc. Natl. Acad. Sci. USA, 78:(1):167-171 (1981).
Sutcliffe, "Complete Nucleotide Sequence of the Escherichia coli Plasmid pBR322", Symp. Quant. Biol. 43:77-90 (1979).
Teather and Wood, "Use of Congo Red-Polysaccharide Interactions in Enumeration and Characterization of Cellulolytic Bacteria from the Bovine Rumen", Appl. Environ. Microbiol., 43:(4):777-780 (1982).
Teeri et al., "The Molecular Cloning of the Major Cellulase Gene From Trichoderma reesei", Bio/Technology (reprint) Oct. 1983.
Vieira and Messing, "The pUC Plasmids, and M13mp7-derived system for insertion mutagenesis and Sequencing with Synthetic Universal Primers", Gene, 19:259-268 (1982).
Watson, "Compilation of Published Signal Sequences", Nucleic Acids Research, 12:(13):5145-5164 (1984).
Wickner and Lodish, "Multiple Mechanisms of Protein Insertion Into and Across Membranes", Science, 230:400-407 (Oct. 1985).
Wong et al., "Characterization and Structure of an Endoglucanase Gene cen A of Cellulomonas fimi", Gene, 44:315-324 (1986).
Maniatis et al. In Molecular Cloning, A Laboratory Manual, Cold Spring Harbor, 1982, p. 143.
Kilburn Douglas G.
Miller, Jr. Robert C.
Warren R. Anthony J.
Fleisher Mindy
Rae-Venter Barbara
Schmickel David
University of British Columbia
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