Chamber for the treatment of cells in an electric field

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435287, 435289, 435817, 204272, C12P 1700, G01N 2728

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active

046998810

ABSTRACT:
The chamber for treating cells in an electric field includes an interior ce for a cell suspension having a region between the electrodes inside of which the cells are exposed to the electric field. In order to provide an improved chamber the region in which the cells are treated--up to an inlet or outlet for the cell suspension--is bounded by surfaces of the electrodes which are positioned equidistant from each other. Preferable these electrode surfaces are coaxial or concentric. In one specific embodiment of my chamber a first electrode is the inner electrode and a second electrode is the outer electrode and the surfaces of the inner electrode and the inside surfaces of the outer electrode are cylindrical so that the region between the electrodes is a cylindrical annular space. The front end of the circular space opens to the exterior while the other opposing end of the circular space opens into an axial duct in the inner electrode which is connected to the inlet of the chamber by a conical passage which widens toward the exterior whose dimensions are selected so that a standard pipette can be inserted into it.

REFERENCES:
patent: 2440472 (1948-04-01), Horner et al.
patent: 2955076 (1960-10-01), Gosshing
patent: 3095359 (1963-06-01), Heller
patent: 3133003 (1964-05-01), Schaefer et al.
patent: 3528905 (1970-09-01), Miller
patent: 4578168 (1986-03-01), Hofmann

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