Chamber and process for crossed immunoelectro-phoresis

Details Chamber and process for crossed immunoelectro-phoresis Chamber and process for crossed immunoelectro-phoresis

1974-12-27

1976-06-22

Mack, John H.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

204180R, 204180G, G01N 2726, G01N 2728

Patent

active

039649920

ABSTRACT:
A chamber and process for performing the electroimmunomigration step (the second phase of a crossed-electrophoresis process) for separating protein-containing biological fluids. The chamber preferably includes a base plate having a well defined therein, a depot subdividing the well into migration and connecting wells, and a device for defining a vertically variable gap in the depot so that a first carrier having a protein-containing sample in the depot is in contact with a second carrier in the migratory well and/or connecting well. Various devices may be provided for particularly defining the gap, and for defining the depot and the areas of the wells adjacent the depot.

REFERENCES:
patent: 3255100 (1966-06-01), Raymond
patent: 3407133 (1968-10-01), Oliva et al.
patent: 3479265 (1969-11-01), Elevitch
patent: 3649499 (1972-03-01), Virtanen et al.
patent: 3766047 (1973-10-01), Elevitch
Groc & Jendrey, "Modifications of Laurell's Crossed Immunoelectrophoresis With Special Gel Chamber," Clinica Chimica Acta, 52, (1974) 59-69.
Giebel and Saechtling "A Combination of Micro-Disc Electrophoresis With Antigen-Antibody Crossed Electrophoresis," Hoppe-Seyler's Z. Physiol. Chem., vol. 354, June, 1973, pp. 673-681.

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