Chain-terminating nucleotides for DNA sequencing methods

Details Chain-terminating nucleotides for DNA sequencing methods Chain-terminating nucleotides for DNA sequencing methods

1993-08-02

1996-08-20

Jones, W. Gary

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 912, 536 2714, 536 282, C12P 1934

Patent

active

055478590

ABSTRACT:
There are provided variant family B DNA polymerases having no 3'.fwdarw.5' exonuclease activity. These variant polymerases have utility as DNA sequencing polymerases. Methods for DNA sequencing with family B DNA polymerases and chain-terminating nucleotides not previously used for sequencing have been developed. The methods disclosed involve the use of family B DNA polymerases not known heretofore to have utility in DNA sequencing, such as variant or wild type forms of phage T4 DNA polymerase or Escherichia coli DNA polymerase II, with novel combinations of deoxynucleotides and chain-terminating nucleotides.

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