Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Reexamination Certificate
2005-08-02
2005-08-02
Prouty, Rebecca (Department: 1652)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
C435S194000, C530S350000, C436S094000, C436S501000
Reexamination Certificate
active
06924119
ABSTRACT:
As a cGMP-visualizing probe capable of detecting and quantifying cGMP easily and accurately even in vivo and for a method of detecting and quantifying cGMP by using the same, a cGMP-visualizing probe comprising a polypeptide, which binds specifically to cGMP, and two chromophores having different fluorescence wavelengths each linked respectively to the two terminals of said polypeptide is provided.
REFERENCES:
patent: 5998204 (1999-12-01), Tsien et al.
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Norbert Wild et al., “Expression of a chimeric cGMP-sensitive regulatory subunit of the cAMP-dependent protein kinase type Iα”, FEBS Letters, 374, 1995, pp. 356-362.
Kazuhiko Seya et al., “A Fluorometric Assay for Cyclic Guanosine 3′, 5′-Monophosphate Incorporating a Sep-Pak Cartridge and Enzymatic Cycling”, Analytical Biochemistry, 272, 1999, pp. 243-249.
Moritoshi Sato et al., “Fluorescent Indicators for Cyclic GMP Based on Cyclic GMP-Dependent Protein Kinase Iα and Green Fluorescent Proteins”, Analytical Chemistry, Dec. 15, 2000, 72, pp. 5918-5924.
Akira Honda et al., “Spatiotemporal dynamics of guanosine 3′, 5′-cyclic monophosphate revealed by a genetically encoded, fluorescent indicator”, PNAS, Feb. 27, 2001, 98, pp. 2437-2442.
Ozawa Takeaki
Sato Moritoshi
Umezawa Yoshio
Japan Science and Technology Corporation
Prouty Rebecca
Wenderoth , Lind & Ponack, L.L.P.
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