Cesium trifluoroacetate solutions for the isolation of nucleic a

Details Cesium trifluoroacetate solutions for the isolation of nucleic a Cesium trifluoroacetate solutions for the isolation of nucleic a

1983-01-17

1984-11-13

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

436542, 436545, 436547, 436804, 536 22, 536 28, 536 29, C07G 700, C07H 1700, C07H 1900, C07H 2100

Patent

active

044824823

ABSTRACT:
A method for simultaneously isolating DNA, RNA and/or protein fractions from a crude biological mixture containing DNA, RNA and/or protein fractions is disclosed. The method comprises adding the crude biological mixture to an aqueous solution of cesium trifluoroacetate, the solution having a density from about 1.4 to about 1.6 g/ml, and centrifuging the resulting mixture under ultracentrifugation conditions.

REFERENCES:
Nucleic Acids Research, 4819-4836 (1978), Burke et al.
Biopolymers-vol. 6, pp. 1325-1344 (1968), Tunis et al.
Biopolymers-vol. 10, pp. 1901-1924 (1971), Schmid et al.
Biopolymers-vol. 6, pp. 1345-1353 (1968), Tunis et al.
Analytical Biochemistry, 86, 264-270 (1978), Burke et al.

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