Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1987-10-23
1991-09-10
Stone, Jacqueline
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435200, 435129, 435134, C12P 1904, C12P 764, C12P 1302, C12N 924
Patent
active
050473379
ABSTRACT:
Ceramide-glycanase which cleaves the entire oligosaccharides intact from glycosphingolipids and alkylglycolipids. The enzyme does not hydrolyze monosaccharides from these glycoconjugates. The enzyme is obtained from the muscle tissue of annelids such as earthworms and leeches by homogenization in water, ammonium sulfate fractionation, gel permeation chromatography, and hydrophobic interaction chromatography, and is essentially free from exo-glycosidase activity. Also disclosed is a method for obtaining oligosaccharides by hydrolysis of glycolipids, alkylglycolipids, and glycosphingolipids by the ceramide-glycanase.
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Li Su-Chen
Li Yu-Teh
Patterson Charles L.
Stone Jacqueline
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