Cephalosporin acetylhydrolase gene from Bacillus subtilis

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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4353201, 4351723, 43525233, 536 232, 935 14, C12N 918, C12N 1500, G07H 1512

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052815250

ABSTRACT:
A cephalosporin acetylhydrolase gene, a recombinant DNA molecule prepared by introducing said gene into a vector used in an E. coli host-vector system, E. coli cells transformed with said recombinant DNA molecule, a protein having the amino acid sequence encoded by said gene, an enzyme which is a multimer of said protein, and a process for preparing said protein or enzyme are provided, said cephalosporin acetylhydrolase being an enzyme useful for converting cephalosporins such as cephalosporin C and 7-ACA into deacetylated ones such as deacetylcephalosporin C and deacetyl 7-ACA which are useful as an intermediate for preparing a variety of derivatized cephalosporin antibiotics.

REFERENCES:
patent: 3304236 (1967-02-01), Nuesch et al.
Suggs et al. PNAS, vol. 78, No. 11, Nov. 1981, pp. 6613-6617.
Konecny et al., Biochimica et Biophysica Acta, vol. 485 (1977) pp. 367-378.
Higerd et al., Journal of Bacteriology, vol. 114, No. 3 1977 pp. 1184-1192.
J. Konecny, Enzymatic Deacetylation of Cephalosporins pp. 253-259.
Abbott et al., Applied Microbiology, vol. 20, No. 3, pp. 413-419 (1975).
Abbott et al., Methods in Enzymology, vol. 43, pp. 731-734 (1975).

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