Cephalosporin acetylhydrolase gene and protein encoded by said g

Details Cephalosporin acetylhydrolase gene and protein encoded by said g Cephalosporin acetylhydrolase gene and protein encoded by said g

1992-11-25

1994-08-16

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

536 232, C12N 918, C12N 1500

Patent

active

053386760

ABSTRACT:
A cephalosporin acetylhydrolase gene, a recombinant DNA molecule prepared by introducing said gene into a vector used in an E. coli host-vector system, E. coli cells transfomed with said recombinant DNA molecule, a protein having the amino acid sequence encoded by said gene, an enzyme which is a multimer of said protein, and a process for preparing said protein or enzyme are provided, said cephalosporin acetylhydrolase being an enzyme useful for converting cephalosporins such as cephalosporin C and 7-ACA into deacetylated ones such as deacetylcephalosporin C and deacetyl 7-ACA which are useful as an intermediate for preparing a variety of derivatized cephalosporin antibiotics.

REFERENCES:
patent: 3304236 (1967-02-01), Nuesch et al.
Konecny et al., Biochimica et Biophysica Acta, vol. 485 (1977) pp. 367-378.
Higerd et al., Journal of Bacteriology, vol. 114, No. 3 1977 pp. 1184-1192.
J. Konecny, Enzymatic Deacetylation of Cephalosporins pp. 253-259.
Abbott et al, Applied Microbiology, vol. 20, No. 3, pp. 413-419 (1975).
Abbott et al, Methods in Enzymology, vol. 43, pp. 731-734 (1975).
Suggs et al. PNAS, vol. 78, No. 11, Nov. 1981, pp. 6613-6617.

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