Centrifuge rotor apparatus for preparing particle spreads

Details Centrifuge rotor apparatus for preparing particle spreads Centrifuge rotor apparatus for preparing particle spreads

1979-02-28

1984-01-03

McIntosh, John P.

Coating apparatus

With means to centrifuge work

118407, B05C 1108

Patent

active

044236999

ABSTRACT:
A centrifuge rotor is described which facilitates the preparation of cell dispersions on microscope slides. The rotor is bowl-like in configuration and defines an annular channel having plural circumferentially disposed septa. The septa provide plural separate regions each adapted to receive removable chambers. The outer wall in each region is flat to accommodate a microscope slide. Each chamber has an outlet orifice adapted to contact the microscope slide. A sample containing blood cells, for example, may be placed in each chamber and the cells centrifugally sedimented against the slide associated with the respective chambers. A gasket at the interface between each slide and its chamber prevents leakage of the sample and a conduit in each chamber permits removal of the supernatant fluid following cell sedimentation.

REFERENCES:
patent: 3352280 (1967-11-01), Hughes et al.
patent: 3619865 (1971-11-01), Hazzard
patent: 3705048 (1972-12-01), Staunton
patent: 3906890 (1975-09-01), Amos et al.
patent: 4093350 (1978-06-01), Fisli
Leif et al., "Centrifugal Cytology IV, The Preparation of Fixed Stained Dispersions of Gynecological Cells", Acta Cytologica, vol. 19, No. 2, Mar.-Apr. 1975, pp. 159-168.
"A Slide Centrifuge: An Apparatus for Concentrating Cells in Suspension onto a Microscope Slide", Watson, Reprinted from The Journal of Laboratory and Clinical Medicine, vol. 68, No. 3, pp. 494-501, Sep. 1966.
Dore et al., A Device for Preparing Cell Spreads, Immunology, Mar. 1965, vol. 9, pp. 403-405.
Dunlap et al., Centrifugal Cylology, The Journal of Histochemistry and Cytochemistry, vol. 23, No. 5, pp. 369-377, 1975.
Leemann et al., A Centrifugation Technique for Cytochemical Preparations, The Journal of Histochemistry and Cytochemistry, vol. 19, No. 12, 1971, pp. 758-760.
Ingram et al., Semiautomatic Preparation of Coverglass Blood Smears Using a Centrifugal Device, The American Journal of Clinical Pathology, vol. 51, No. 2, 1969, pp. 214-221.
Lee et al., Automated System for Fractionation of Blood Samples, Clinical Chemistry, vol. 24, No. 8, 1978, pp. 1361-1365.

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