Chemistry: analytical and immunological testing – Including sample preparation – Liberation or purification of sample or separation of...
Patent
1993-02-24
1997-03-11
Beisner, William
Chemistry: analytical and immunological testing
Including sample preparation
Liberation or purification of sample or separation of...
436178, 536 254, 435325, 435261, 435410, 4353061, 4353081, 422 72, 210787, 2105121, 935 19, 494 17, 494 29, 494 37, G01N 118, C07H 2102, C12M 112, C12N 102
Patent
active
056100744
ABSTRACT:
A centrifugal rotor effects the isolation, in a sequence of steps, of a substance from a mixture of substances dissolved, suspended or dispersed in a sample liquid. Multiple samples are processed simultaneously by means of a plurality of fractionation cells, each of which contains a series of interconnected, chambered and vented compartments in which individual steps of the fractionation and isolation procedure take place. Specific steps in the preferred embodiments include lysis, sedimentation, aggregation, sorption, rinsing, and desorption. The specific compartment occupied by the sample liquid or one of its fractions at any stage of the process is governed by the speed and direction of rotation of the rotor and by gravitational force. The interconnections, chambers and passages of each compartment are sized and angled to prevent predetermined amounts of sample and reagent liquids from overflowing the compartment. Predetermined amounts of the reagent liquids required to contact the sample in individual steps of the isolation process are simultaneously delivered to specific compartments of every cell by means of a dynamic flow distributor which accepts partially aerosolized injections of said reagents pipetted from a dosator while the centrifugal rotor is rotating. A method of using the rotor to isolate deoxyribonucleic acid is described. The operation of the centrifuge and the dosator can be controlled and coordinated automatically.
REFERENCES:
patent: 2834541 (1956-09-01), Szent-Gyorgi et al.
patent: 3235173 (1966-02-01), Unger
patent: 3379370 (1966-09-01), Anderson
patent: 3586484 (1969-05-01), Anderson
patent: 3722789 (1972-01-01), Kennedy
patent: 3873217 (1973-07-01), Anderson et al.
patent: 3901658 (1974-07-01), Burtis et al.
patent: 4244916 (1981-01-01), Guigan
patent: 4422941 (1983-12-01), Vaughan et al.
patent: 4456581 (1984-06-01), Edelmann et al.
patent: 4463097 (1984-07-01), Guigan
patent: 4469793 (1984-09-01), Guigan
patent: 4519981 (1985-05-01), Guigan
patent: 4663296 (1987-05-01), Revillet et al.
patent: 4714590 (1987-12-01), Guigan
patent: 4756883 (1988-07-01), Romanauskas
patent: 4787971 (1988-11-01), Donald
patent: 4788154 (1988-11-01), Guigan
patent: 4812294 (1989-03-01), Combs
patent: 4832851 (1989-05-01), Bowers et al.
patent: 4900446 (1990-02-01), Anderson
patent: 4999304 (1991-03-01), Robertson
patent: 5032288 (1991-07-01), Columbus et al.
patent: 5039401 (1991-08-01), Columbus et al.
patent: 5045047 (1991-09-01), Hutchins et al.
patent: 5061381 (1991-10-01), Burd
patent: 5077013 (1991-12-01), Guigan
patent: 5124041 (1992-06-01), Sheer et al.
patent: 5186824 (1993-02-01), Anderson et al.
Beritashvili David R.
Ershov Gennady M.
Georgiev George P.
Kapanadze George I.
Myakishev Maxim V.
Beisner William
Fitting Thomas
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