Centrifugal adsorptive sample preparation device and method

Details Centrifugal adsorptive sample preparation device and method Centrifugal adsorptive sample preparation device and method

1996-03-29

1998-11-10

Fortuna, Ana

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210767, 21032175, 2105021, 210490, 21050036, 210473, 210477, 2103601, 2103801, 156 9, B01D 6100

Patent

active

058338608

ABSTRACT:
Adsorptive sample preparation device and method effective for concentrating, desalting and/or purifying biomolecules. An adsorptive membrane is used to bind biomolecules, which biomolecules can then be eluted with a suitable desorption agent. In a preferred form, the adsorptive filter means is a particle-laden semipermeable membrane having at least one of its major surfaces, preferably both of its major surfaces, covered with a thin fabric. In the method of the invention for concentrating biomolecules or desalting or purify from a solution, the sample reservoir of the above-described adsorptive sample preparation device is filled with an analyte solution, placed in a fixed angle or swinging bucket centrifuge rotor, the solution is driven through the membrane, and analyte obtained as a result of adsorption on the membrane is recovered by washing and then eluting with a suitable desorption agent. A method of sealing the membrane in the device is also set forth.

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