Cellulose synthase gene

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435194, 4353201, 4352523, 536 232, C12P 1904, C12N 912, C12N 1554

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060514078

ABSTRACT:
mRNA was extracted at the stage for cotton plant fibrous cells to accumulate cellulose, and cDNA's complementary thereto were synthesized to construct a cDNA library. Clones of a number of 750 were arbitrarily selected from the library, and they were randomly subjected to sequencing. Those having homology to an amino acid sequence deduced from a gene of cellulose 4-.beta.-glucosyltransferase (bcsA) of cellulose synthase operon of acetic acid bacterium were selected from obtained nucleotide sequences of the respective clones. Thus, DNA coding for cellulose synthase was obtained.

REFERENCES:
patent: 5268274 (1993-12-01), Ben-Bassat et al.
GenBank Database Accession U58283 "Gossypium hirsutum cellulose synthase (celA1) mRNA, complete cds" Submitted by Pear et al, Dec. 5, 1996.
GenBank Database Accession U58284 "Gossypium hirsutum cellulose synthase (celA1) mRNA, complete cds" Submitted by Pear et al, Dec. 5, 1996.
Pear, J. R. et al.; "Higher Plants Contain Homologs of the Bacterial CelA Genes Encoding the Catalytic Subunit of Cellulose Synthase", Proc. Natl.Acad.Sci.USA, vol. 93, Oct. 1996, pp. 12637-12642.
Li, L., et al.; "B-Glucan Synthesis in the 1-4 Cotton Fiber", Plant Physiology, vol. 101, No. 4, 1993, pp. 1149-1156.

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