Cellulose synthase associated proteins

Details Cellulose synthase associated proteins Cellulose synthase associated proteins

1992-10-06

1995-01-17

Wax, Robert A.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

435194, 435823, C07K 1504, C12N 912

Patent

active

053826566

ABSTRACT:
Novel proteins capable of binding to bacterial cellulose synthase are provided. Proteins that co-purify with bacterial cellulose synthase and have approximate relative molecular weights of 20 kDa, 54 kDa and 59 kDa are taught. Also provided for are nucleotide sequences encoding the proteins, antibodies to the proteins, and recombinant host cells for the expression of the cellulose synthase associated (co-purifying) proteins. The cellulose synthase associated proteins (CSAPs) CSAP20 (SEQ ID NO: 1) CSAP54 (SEQ ID NO: 2), and CSAP59 (SEQ ID NO: 3) are specifically provided for.

REFERENCES:
Lin, F. C., et al., in Proceedings of the Tenth Cellulose Conference, p. 27, Jun. 1988.
Saxena, I. M., et al., Plant Molecular Biology, vol. 15, No. 5, pp. 673-683, Nov., 1990.
Saxena, I. M. et al., Plant Molecular Biology, vol. 16, No. 6, pp. 947-954, Jun., 1991.
Cannon, R. E., et al., Microbiology 1991, pp. 435-447, 1991.
Mayer, et al., "Polypeptide composition of bacterial cyclis diguanylic acid-dependent cellulose synthase and the occurrence of immunologically crossreacting proteins in higher plants", Proc. Natl. Acad. Sci. USA 88: 5472-5476 (1991).
Lin & Brown, "Purification of Cellulose Synthase from Acetobacter Xylinum", Cellulose and Wood, Conrad Schuerch, Editor, Wiley, N.Y. pp. 473-492 (1989).
Wong, et al., "Genetic organization of the cellulose synthase operon in Acetobacter xylinum", Proc. Natl. Acad. Sci. USA 87: 8130-8134 (1990).
Ross, et al., "Cellulose Biosynthesis and Function in Bacteria", Microbiological Reviews 55: 35-58 (1991).

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