Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1990-10-25
1993-04-13
Naff, David M.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 691, 435 6952, 435 697, 435 711, 4351723, 435177, 435179, 435200, 435209, 435803, 530808, 530814, 935 11, 935 14, C12N 914, C12N 1112, C12N 1500, C12P 2100
Patent
active
052022471
ABSTRACT:
A fusion protein is prepared containing a polypeptide such as an enzyme and an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that has essentially no polysaccharidase activity. By contacting the fusion protein with an affinity matrix containing a substrate such as cellulose for the cellulase substrate binding region, the substrate binding region binds to the affinity matrix to immobilize the polypeptide. The polypeptide can be purified by separating the fusion protein or polypeptide from the affinity matrix. The polypeptide can be separated by cleaving the protein with a Cellulomonas fimi protease.
REFERENCES:
patent: 4745055 (1988-05-01), Schenk et al.
Svensson, et al., "Characterization of a glucoamylase G2 from Aspergillus niger," Eur. J. Biochem., vol. 154, pp. 497-502 (1986).
Takahashi, et al., "Different Behavior towards Raw Starch of Three Forms of Glucoamylase from a Rhizopus Sp.", J. Biochem, vol. 98, pp. 663-671 (1985).
"Sequence homology between putative raw-starch binding domains from different starch-degrading enzymes," Biochem J., vol. 264, pp. 309-311 (1989).
Watanabe, et al., "Gene Cloning of Chitinase Al from Bacillus circulans WL-12 Revealed Its Evolutionary Relationship to Serratia Chitinase and to the Type III Homology Units of Fibronectin," The Journal of Bio. Chem., vol. 265, pp. 15659-15665 (1990).
Watanabe, et al., "Chitinase System of Bacillus circulans WL-12 and Importance of Chitinase A1 in Chitin Degradation," Journal of Bacteriology, vol. 172, pp. 4017-4022 (1990).
Kellett, et al., "Xylanase B and an arabinofuranosidase from Pseudomonas fluorescens subsp. cellulosa contain identical cellulose-binding domains and are encoded by adjacent genes", Biochem. J., vol. 272, pp. 369-376 (1990).
"Purification and Characterization of Endoglucanase C of Cellulomonas fimi, Cloning of the Gene, and Analysis of In Vivo Transcripts of the Gene", by Moser et al. in Applied and Environmental Microbiology, 55:2480-2487 (1989).
"Precise Excision of the Cellulose Binding Domains from Two Cellulomonas fimi Cellulases by a Homologous Protease and the Effect on Catalysis", by Gilkes et al. in The Journal of Biological Chemistry, 263:10401-10407 (1988).
"Regulation and Initiation of cenB Transcripts of Cellulomonas fimi", by Greenburg et al. in Journal of Bacteriology, 169:4574-4677 (1987).
"Expression in Escherichia coli of the Cellulomonas fimi Structural Gene for Endoglucanase B", by Owolabi et al. in Applied and Environmental Microbiology, 54:518-523 (1988).
Gilkes Neil R.
Kilburn Douglas G.
Miller Robert C.
Warren Richard A. J.
Naff David M.
Rae-Venter Barbara
University of British Columbia
LandOfFree
Cellulose binding fusion proteins having a substrate binding reg does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Cellulose binding fusion proteins having a substrate binding reg, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Cellulose binding fusion proteins having a substrate binding reg will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1154816