Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1988-07-08
1992-08-11
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435 691, 435 6952, 435 697, 435 711, 4351723, 435177, 435195, 435200, 435209, 435803, 935 11, 935 14, C12N 1112, C12N 1500, C12P 2100
Patent
active
051378191
ABSTRACT:
A fusion protein is prepared containing a polypeptide such as an enzyme and an amino acid sequence having a substrate binding region of a polysaccharidase such as cellulase that has essentially no polysaccharidase activity. By contacting the fusion protein with an affinity matrix containing a substrate such as cellulose for the cellulase substrate binding region, the substrate binding region binds to the affinity matrix to immobilize the polypeptide. The polypeptide can be purified by separating the fusion protein or polypeptide from the affinity matrix.
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Gilkes Neil R.
Kilburn Douglas G.
Miller Robert C.
Warren Richard A. J.
Naff David M.
Rae-Venter Barbara
University of British Columbia
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