Cellulase isolated from Bacillus ferm BP-3431 or a mutant strain

Details Cellulase isolated from Bacillus ferm BP-3431 or a mutant strain Cellulase isolated from Bacillus ferm BP-3431 or a mutant strain

1991-07-23

1993-07-27

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435832, 43525231, C12N 942, C12N 122, C12N 100

Patent

active

052310221

ABSTRACT:
A cellulase having the following properties: (1) an optimum pH of from 9.5 to 10.5 as measured using carboxymethylcellulose as a substrate; (2) a stable pH of from 6 to 11 as measured using carboxymethylcellulose as a substrate after treating at 30.degree. C. and for 30 minutes; (3) an optimum temperature of about 55.degree. C. as measured using carboxymethylcellulose as a substrate; (4) influence of a surfactant being such that residual activity is 90% or more after treatment at 30.degree. C. and pH of 9.0 for 2 hours in the presence of a sodium n-alkylbenzenesulfonate; (5) a molecular weight of 52,000.+-.2,000 as measured by electrophoresis on SDS-polyacrylamide gel; and (6) an isoelectric point of 4.2.+-.0.2 as measured by isoelectric electrophoresis. The cellulase is produced by Bacillus sp. SD402, its mutant strains or its genetic engineered strains. An aid for detergents and a paper treating agent, respectively, contain the cellulase as an effective ingredient.

REFERENCES:
patent: 3844890 (1974-10-01), Horikoshi et al.
patent: 3983002 (1976-09-01), Ohya et al.
patent: 4945053 (1990-07-01), Ito et al.
patent: 4962030 (1990-10-01), Kawai et al.
patent: 5045464 (1991-09-01), Ito et al.
J. Pommier et al., "Using Enzymes to Improve the Process and the Product Quality in the Recycled Paper Industry", Tappi Journal 73 (12), pp. 197-202, 1990.
J. Pommier et al., "Using Enzymes to Improve the Process and the Product Quality in the Recycled Paper Industry", Tappi Journal 72 (6), 197-201, 1989.
Y. Yoneda et al., "Mutation of Bacillus Subtilis Causing Hyperproduction of .alpha.-Amlase and Protease, and Its Synergistic Effect", Journal of Bacteriology 124 (1), pp. 48-54, 1975.
R. Teather et al., "Use of Congo Red-Polysaccharide Interactions in . . . Bovine Rumen", Applied & Environmental Microbiology 43, pp. 777-780, 1982.
K. Ozaki et al., "Molecular Cloning and Nucleotide Sequence of a Gene for Alkaline Cellulase from Bacillus sp. KSM-635", Journal of General Microbiology 136, pp. 1327-1334, 1990.
C. Huang et al., "Molecular Cloning and Expression of Multiple Cellulase Genes of . . . Coli", Applied & Microbiology Biotechnology 31, pp. 265-271, 1989.
F. Fukumori et al., "Purification and Properties of a Cellulase from Alkalophilic Bacillus sp. No. 1139", Journal of General Microbiology 131, pp. 3339-3345, 1985.
S. Shikata et al., "Alkaline Cellulases for Laundry Detergents: Production by . . . Enzymes", Agric. Biol. Chem. 54 (1), pp. 91-96, 1990.

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