Cellulase-free endo-xylanase enzyme of use in pulp delignificati

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 691, 435 711, 435 91, 435169, 4351721, 435200, 4352523, 43525235, 4353201, 435886, 536 27, 162 1, 935 6, 935 9, 935 14, 935 22, 935 59, 935 60, 935 64, 935 72, D21C 300, C12N 121, C12N 1552, C12N 1576

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051167468

ABSTRACT:
A method of treating lignocellulosic material with a cellulase-free endo-xylanase for delignification, brightening and viscosity improvement. The endo-xylanase is obtained by the overexpression of the xylanase gene using a cellulase-negative recombinant microorganism of the genus Streptomyces. The recombinant microorganism is produced in a homologous cloning system in which the xylanase gene is inserted into a vector plasmid to provide the hybrid plasmid that is introduced into a host cellulase-negative mutant strain and said xylanase gene, said vector plasmid and said host mutant strain are obtained from microorganisms of the genus Streptomyces.

REFERENCES:
Bernier, R., Driquez, H. and Desrochers, M., Gene; 26, 59 (1983).
Iwasaki, A., Kishida, H. and Okanishi, M., J. Antibiotics: 39(7),985 (1986).
Viikari, L., Ranua, M., Kantelinen, A., Linko, M. and Sundquist, J., Proceedings of the International Symposium on Wood & Pulping Chemistry, Paris (1987).
Mondou, F., Shareck, F., Morosoli, R. and Kluepfel, D., Gene; 49(3), 323 (1986).
Chauvet, J-M., Comtat, J. and Noe, P., Proceedings of the International Symposium on Wood & Pulping Chemistry, Paris (1987).

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