Cellular uptake of modified peptides

Details Cellular uptake of modified peptides Cellular uptake of modified peptides

1996-05-03

1998-04-07

Huff, Sheela

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

530330, 530345, 436 64, 436176, 4351731, 435184, 435963, 435966, C12N 500, C07K 500, C07K 700, C07K 1700

Patent

active

057363943

ABSTRACT:
Disclosed herein is a cell containing a modified peptide. More specifically, the N-terminal amino acid residue of the peptide is modified by the addition of an aryl ketone group which, when contacted with an appropriate substrate, and exposed to light having a wavelength of about 330 nm or greater, results in the covalent bonding of the peptide to the substrate by a C--H insertion dominant mechanism. In preferred, embodiments, the aryl ketone is a benzophenone moiety. The peptide can be designed to specifically bind to a protein of interest in the cell. The cell is then contacted with light having a wavelength of greater than about 330 nm to bind the peptide covalently to the binding site on the intracellular protein of interest. In this way, the modified peptide can be used to specifically and irreversibly block a binding site on an intracellular protein of interest.

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Nakamoto et al, Biochemistry 34: 10546 (1995).
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