Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-05-10
1997-10-14
Sisson, Bradley L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 6, 4352402, 4353201, 435183, 435270, 536 235, 935 1, 935 16, 935 22, 935 76, 935 77, C12P 2106, C12N 108, C12N 1500, C07H 2104
Patent
active
056771436
ABSTRACT:
The invention relates to a cellular protein which is specific and has high affinity for nucleic acid sequences characteristic of an intact TAR RNA loop sequence of the HIV LTR TAR region. The invention also relates to a protein preparation having a protein of about 185 kD that is isolated from a mammalian cell nuclear extract preparation, most specifically a HeLa cell extract that is purified between 1,000-10,000 fold. The protein of about 185 kD is shown to regulate HIV viral gene expression by binding a TAR RNA region of an HIV LTR template, in the presence of a cofactor fraction (including at least a -100 kD cofactor), and a tat protein. A route for the development of immunodiagnostics for AIDS and related disorders may also be provided given the specific and high affinity of TRP-185 for HIV RNA. The 185 kD protein and related encoding amino acid and DNA sequences are provided and may be used in therapeutic agents for AIDS and related disorders and, for the generation of specific antibodies for use in the diagnosis and study of the epidemiology of AIDS.
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Gaynor Richard B.
Wu Foon K.
Board of Regents, University of TX System
Mayfield Denise L.
Sisson Bradley L.
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