Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1987-06-22
1990-09-25
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 701, 4351721, 4351723, 435320, 935 6, 935 33, 935 36, 935 34, 536 27, C12N 1500, C12P 2100, C07H 1512
Patent
active
049593137
ABSTRACT:
A novel cellular enhancer nucleotide sequence causes expression in undifferentiated stem cells of a flanking exogenous or recombinant gene from a promoter accompanying the gene where the gene and promoter are not normally expressed in the undifferentiated stem cells. In the preferred example the essential or basic core of the cellular enhancer nucleotide sequence is ##STR1## The cellular enhancer may encompass a more general core sequence of approximately, for example, in the range of 300 to 350 bases including the essential or basic core sequence. Recombinant vectors including plasmids and viruses are constructed bearing the novel cellular enhancer flanking a recombinant or exogenous gene and promoter having a specified phenotypic trait to be expressed in undifferentiated stem cells. Propagating cells containing the vector constructs reproduce and propagate the vectors. Undifferentiated stem cells including cell lines and animals express the desired phenotypic trait of an exogenous or recombinant gene under the control of the novel cellular enhancer.
REFERENCES:
patent: 4663281 (1987-05-01), Gillies et al.
Linney et al (1983), Cell 35:693-699.
Sorge et al (1984), PNAS 81:6627-6631.
Taketo, M. et al, "Isolation of Embryonal Carcinoma Cell Lines That Express Integrated Recombinant Genes in Undifferentiated Embryonic Stem Cells", Proc. Natl. Acad. Sci. USA, vol. 82, pp. 2422-2426 (1985).
Taketo, M. et al., "A Cellular Enhancer of Retrovirus Gene Expression in Embryonal Carcinoma Cells," Proc. Natl. Acad. Sci. USA, vol. 84 (1987).
Brown Anne
Kane Daniel H.
Schwartz Richard A.
The Jackson Laboratory
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