Cellular assays for identifying signaling peptides

Details Cellular assays for identifying signaling peptides Cellular assays for identifying signaling peptides

2005-03-15

2005-03-15

Brumback, Brenda (Department: 1647)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S069100, C435S069800, C435S069700, C435S070100, C435S320100, C435S325000, C536S023500, C536S024100

Reexamination Certificate

active

06866998

ABSTRACT:
The invention includes a DNA encoding a known translocatable protein, for example, human mpl, lacking secretory ability due to deletion of its secretory signal and the major part of the extracellular region thereof. This DNA is present in an expression vector into which a test cDNA from a cDNA library is ligated. The expression vector containing the known DNA and the test cDNA expresses a chimeric protein encoded by these two nucleic acid molecules. Cells expressing the newly constructed vector are capable of proliferating if the test cDNA is a secretory protein. Thus, cDNAs encoding secretory proteins including type I membrane proteins and type II membrane proteins can be detected and isolated by constructing a cDNA library and screening it for ability to promote cell proliferation.

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