Cells transformed or transfected with HCMV US2-US5, US10-US11 ge

Details Cells transformed or transfected with HCMV US2-US5, US10-US11 ge Cells transformed or transfected with HCMV US2-US5, US10-US11 ge

1997-10-07

1999-05-25

McKelvey, Terry

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

435243, 4353201, 435410, C12N 510, C12N 100, C12N 1579

Patent

active

059069350

ABSTRACT:
Infection of human fibroblast cells with human cytomegalovirus (HCMV) causes down regulation of cell surface expression of MHC class I. The present invention is directed to a mutant with a 9-kb deletion in the S component of the HCMV genome (including open reading frames IRS1-US9 and US11) which failed to down regulate class I heavy chains. By examining the phenotypes of mutants with smaller deletions with this portion of the HCMV genome, a 7-kb region containing at least 9 open reading frames was shown to contain the genes required for reduction in heavy chain expression. Furthermore, it was determined that two subregions (A and B) of the 7-kb region each contained genes which were sufficient to cause heavy chain down regulation. In subregion B, the US11 gene product is involved. It encodes a endoglycosidase H-sensitive glycoprotein which is intracytoplasmic, similar to the adenovirus type 2 E3-19K glycoprotein which inhibits surface expression of class I heavy chains.

REFERENCES:
patent: 4689225 (1987-08-01), Pereira
patent: 5273876 (1993-12-01), Hock et al.
Marshall, Science, 269, 1995, 1050-1055.
Miller et al., FASEB J., 1995, 9, 190-199.
Culver et al., TIG, 10(5), 1994, 174-178.
Hodgson et al., Exp. Opin. Ther. Pat., 1995, 5(5), 459-468.
Kollert-Jons, J. Virology, 65(10), 1991, 5184-5189.
Jones et al., J. Virology, 65(11), 1991, 5860-5872.
Jones et al., J. Virology, 65(4), 1991, 2024-2036.
NIH Report to Gene Therapy Committee, Dec. 1995.
Jones, T.R. et al., J. Virology, vol. 66, No. 4, 1992, pp. 2541-2546.
Chee M.S. et al., Current Topics in Microbiology and Immunology, vol. 154, 990, pp. 126-169.
Gilbert, M.J. et al., J. Virology, vol. 67, No. 6, 1993, pp. 3461-3469.
Beersma, M.F.C. et al., J. Immunology, vol. 151, No. 9, 1993, pp. 4455-4464.
Jones, T.R. et al., J. Virology, vol. 69, No. 8, 1995, pp. 4830-4841.
Colberg-Poley, A. M. et al., J. Virology, vol. 66, No. 1, 1992, pp. 95-105.

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